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Supplier: EDVOTEK

The second assay used to confirm a positive HIV ELISA result is the Western blot. Using this kit, students separate protein samples from hypothetical patients on agarose gels. The proteins are then transferred to a membrane and simulated HIV proteins are detected.

Supplier: EDVOTEK

Students learn the basic principles of the Enzyme-linked Immunosorbent Assay (ELISA) using this precise and sensitive antibody-based detection kit.

Supplier: EDVOTEK

Infectious agents such as bacteria and viruses can spread rapidly through a population and cause widespready disease and death. In this experiment, students use coloured solutions to simulate the spreading of a disease in the classroom.

Supplier: EDVOTEK

An infectious outbreak requires prompt and accurate identification of the biological agent. Often, early clinical symptoms are first identified in exposed individuals and then infectious agents are identified by lab tests. In this experiment, students transmit a simulated infectious agent (chemical dye) between classmates which is only visible under long UV light. The pattern of transmission and primary source is documented.

Supplier: EDVOTEK

Observe the differences between mammalian cell types and how these cells funciton. Cells are fixed on microscope slides and students stain the cells on the slide to view morphological characteristics of the cell types. These cells are very safe for classroom use.

Supplier: EDVOTEK

Infectious mononucleosis is commonly known as the 'kissing disease'. The causative agent is Epstein-Barr virus (EBV), which can be transmitted through saliva during kissing. In this experiment, students search for the presence of EBV using the ELISA reaction to detect specific viral proteins.

Supplier: EDVOTEK

This experiment introduces students to Southern blotting as a tool for DNA fingerprinting in a hypothetical paternity determination. DNA fragments are first separated by agarose gel electrophoresis, then transferred to a nylon membrane and finally visualised by staining.

Supplier: G-Biosciences

Following restriction enzyme digestion of DNA molecules, researchers need to rejoin the ends of the DNA to generate recombinant DNA, a process known as ligation. Ligation of DNA is achieved with the bacterial enzyme T4 DNA ligase, which catalyses the formation of phosphodiester bonds. The DNA Ligation kit teaches students about ligation as they ligate several DNA fragments together to make larger pieces of DNA that are easily identified by agarose electrophoresis.

Supplier: EDVOTEK

This experiment uses PCR to amplify a small section of Lambda DNA via a 2-step process, saving valuable classroom time and allowing completion of the lab in one session.

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This experiment introduces students to the use of DNA fingerprinting in a simulated paternity determination. A child's DNA fingerprint is compared with his parents.

Supplier: EDVOTEK

The Green Fluorescent Protein (GFP) from the jellyfish Aequorea victoria is used extensively in all areas of science. Many organisms have been transformed with the GFP gene, the gene responsible for bioluminescence in jellyfish. It has proven to be so useful that scientists have mutated it to produce Blue Fluorescent Protein (BFP). In this simple experiment, students transform bacteria either by GFP, BFP or both.

Supplier: EDVOTEK

Four experimental modules are combined into one experiment to provide a comprehensive biotechnology exploration focusing on the green fluorescent protein (GFP). Bacterial cells are transformed to express the GFP. The transformed cells are then grown and the GFP purified by column chromatography. Finally, the purity of the protein fractions are analysed by SDS polyacrylamide electrophoresis.

Supplier: EDVOTEK

When bacteria are used to make medicinally useful proteins by transformation, the protein of interest must be separated from all of the other cellular proteins. In this experiment, the unique fluorescent properties of GFP and BFP are used as an assay during their purification from an E. coli extract. The column fractions containing GFP or BFP are identified by fluorescence and then purified. As an optional activity, purified protein fractions can be separated by SDS polyacrylamide gel electrophoresis (SDS-PAGE) to estimate the purity and size of the GFP and BFP proteins.

Supplier: G-Biosciences

Determination of protein concentration is an essential technique in all aspects of protein studies and proteomics. The Assays for Protein Quantification kit includes three of the most widely used protein assays and allows for a direct comparison of the three assays that teaches students the benefits and limitations of each assay. Each assay is available individually to allow teaching of a specific assay, without the option of comparing and contrasting with other assays. The three assays covered are the Biuret Protein Assay, Lowry Protein Assay and the Coomassie Blue Dye Protein Assay. The Assays for Protein Quantification kit provides all the reagents required to perform both protein assays, including protein standards for accurate quantification, in a single lab activity. An often underestimated factor in quantifying protein is the presence of non-protein interfering agents, such as salts and detergents. This kit teaches students about common laboratory agents that affect the protein assays, the reasoning behind their interferences and how to overcome the interference. Students also learn how to select a protein assay for different applications.

Supplier: G-Biosciences

This lab activity involves analysis of an enzyme reaction using a specific enzyme substrate and inhibitor. Students study how the rate of enzyme reaction is dependent on substrate concentration and the influence of agents (inhibitors) and conditions that affects the enzyme reaction such as pH and temperature.

Supplier: G-Biosciences

Biodiversity lab activity is designed for determination of protein contents and biomass in diverse biological samples to study how biomass is related to biodiversity in nature. Students collect and catalogue plant leaf samples from a diverse group of locally available plants. This lab activity involves determination of natural weight of each plant sample, grinding a predetermined amount of each sample, and the subsequent extraction of proteins from the samples. Students then learn to determine protein contents of each plant sample and attempt to relate the protein content with biomass. Students in this lab activity are challenged to think, analyse, and seek answers as to why protein biomasses vary for a given natural weight for different plants. Finally, they will relate that finding to the biodiversity of nature.

Supplier: G-Biosciences

The Size Exclusion Chromatography kit teaches gel filtration or size exclusion chromatography and the use of this method in purification of biological samples. This method is based on separation of protein molecules based on their molecular size. This lab activity involves running size exclusion chromatography for separation of molecules based on their molecular sizes.

Supplier: G-Biosciences

Bacterial conjugation is a naturally occurring process that allows the transfer of DNA from one bacterium to another, which allows the transfer of genetic traits, particularly drug resistance. The kit contains two bacteria with different drug resistance genes and all the tools for students to study bacterial conjugation. This kit teaches the difference between bacterial genomic DNA and the transferable plasmid DNA and the mechanisms of bacterial conjugation. Students will also learn important basic microbiological techniques, including bacterial growth in liquid broth and on solid agar plates, antibiotic selection of bacteria and important aseptic techniques.

Supplier: MACHEREY-NAGEL

Advanced kit for science education.

Supplier: EDVOTEK

Kit for teaching students how to use a micropipette with ease and accuracy using multi-coloured dyes.

Supplier: EDVOTEK

With this kit, students sample water and air and then grow any microbes present overnight. A safe and simple way to teach pollution.

Supplier: EDVOTEK

Safe drinking water is vitally important to health. Both pathogenic and harmless bacteria can be found in the guts of mammals and birds. Testing water for every possible type of pathogenic bacteria is slow and costly. Thus, water is tested for a characteristic type of gut bacteria - the coliforms - including the familiar E. coli. Presence of coliforms is an indicator for faecal contamination. In this experiment, students test for coliforms in simulated contaminated water using colour and fluorescent reagents. They can use these same reagents to test water samples from the environment. As an extension activity, a Gram Stain test can be performed on the collected samples.

Supplier: G-Biosciences

The Affinity Chromatography kit teaches the basic principle of affinity chromatography utilising highly specific affinity columns. This lab activity involves preparation of a crude protein extract and running affinity exchange chromatography for isolation of a protein.

Supplier: G-Biosciences

In this lab activity, students learn to perform simple protein isolation procedures to isolate the protein fingerprint from various fresh tissues. They will compare the protein fingerprints of 4 different tissues to understand that the function of a particular organ is due to the proteins that are localised to the specific organ. Also included in this kit are four dried protein samples (from mouse liver, brain, heart and lungs) to compare as a control, if fresh tissues cannot be obtained.

Supplier: G-Biosciences

This lab activity has two objectives - to deepen the understanding of protein molecules and for students to learn the potential of electrophoresis in protein analysis. Students study the fundamentals of protein structure from their primary structure to the more complex tertiary and quaternary structures, utilising protein electrophoresis. Complex mixtures of protein samples and characterised pure protein samples, in conjunction with electrophoresis, are utilised to study protein structure and the potential of protein electrophoresis. Using non-denaturing and denaturing electrophoresis, students understand the difference between primary, tertiary and quaternary structures, the importance of disulfhydryl bridges in maintaining protein structure and electrophoresis in studying complete proteins and protein subunits.

Supplier: G-Biosciences

Ion exchange chromatography is used to separate charged molecules from complex biological samples. The charged molecules bind to a solid support carrying an opposite charge to the molecule. Proteins contain regions of charged groups on the surface which interact with the ion exchange groups immobilised on the solid support (resin column). Immobilised proteins are eluted by changing either pH or the salt gradient or a combination of both. This lab activity involves preparation of a crude protein extract and running ion exchange chromatography for isolation of proteins.

Supplier: EDVOTEK

Cancer cells differ from normal cells by the combinations of proteins that are present on their surfaces. Antibodies against these proteins will specifically bind to cancer cells and not to normal cells. This allows early detection of cancer and potentially a way of delivering cancer therapies. In this simulation experiment the reaction of cancer cell markers and their corresponding antigens are demonstrated.

Supplier: EDVOTEK

According to the World Health Organisation, between 2 and 3 million cases of skin cancer occur globally every year. Many of these cases are caused by preventable damage to DNA by UV light. In this experiment, students expose plasmid DNA to shortwave UV light to simulate the effect of sunbathing. The DNA is then analysed by agarose gel electrophoresis to observe the damage.

Supplier: EDVOTEK

When normal cells are grown in culture they stop growing when they become overcrowded (contact inhibition). Cancer cells in culture grow in an uncontrolled way because they have lost this property. This helps tumours to form in the body. In addition, many different cell types can be present in a single tumour. This experiment allows students to see the differences between normal and cancer cells in both their growth and cell types.

Supplier: EDVOTEK

The body defends itself from attack by infectious agents like bacteria and viruses by producing antibodies. Enzyme linked immunosorbent assays (ELISA) test for antibodies present in the blood, which indicate infection. In this experiment, students perform a simulated ELISA test to identify infected samples and compare them to control samples.