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Supplier: G-Biosciences

This lab activity is designed to demonstrate the different classes of protein molecules and their classification based on solubility. Students learn fractionation of soluble, insoluble membrane proteins, and cytoskeleton proteins from a tissue sample. The insoluble protein fraction is further fractionated into hydrophilic and hydrophobic membrane proteins. Cell membrane structure and the role of hydrophobic membrane proteins are considered. This lab activity also provides an opportunity to understand characteristics of various classes of detergents and the role of detergents in solubilisation of hydrophobic membrane proteins.

Supplier: G-Biosciences

The physical properties of proteins kit is a lab activity that enables students to investigate the physical properties of several different proteins. Students will learn about protein solubility and how it is affected by various parameters; including temperature, pH, salt and dielectric constant. They will understand about protein precipitation due to pH, high salt and in the presence of organic solvents and about protein denaturation as a result of high temperature. In addition, the kit will demonstrate how non-protein agents, such as detergents drastically alter the physical properties of protein molecules and as a result, understand the importance of detergents in protein solubilisation. This lab activity involves analysis of three different types of pure proteins and then students alter some of those properties with a detergent and re-examine physical properties of those proteins. Students are challenged to consider how physical properties of protein molecules can be exploited for purification and characterisation of proteins and apply their findings on a test sample of complex tissue extract.

Supplier: EDVOTEK

Plasmid and lambda DNA are pre-digested with restriction endonucleases that recognise and cut double-stranded DNA within or near defined base sequences. Digests are separated by agarose gel electrophoresis.

Supplier: EDVOTEK

This easy and safe experiment allows students to learn about enzyme catalysis, the nature of enzyme action, and protein structure-function relationship. Students will perform an enzyme assay and determine the rate of the enzymatic reaction.

Supplier: EDVOTEK

Small-scale, rapid isolation of plasmid DNA is a routine procedure used for screening and analysis of recombinant DNAs in cloning and sub-cloning experiments. In this experiment, students isolate plasmid DNA without the use of toxic chemicals such as phenol or chloroform.

Supplier: EDVOTEK

Solve the mystery of two boys separated from their parents a decade ago. Their biological mother is identified by mitochondrial DNA and their biological father from chromosomal DNA.

Supplier: EDVOTEK

The DNA from bacteriophage Lambda is a well-characterised linear molecule containing six recognition sites for Eco RI (5 distinct sites; 2 are very close in size). In this experiment, Lambda DNA is digested by the Eco RI endonuclease. The digestion products are analysed by agarose gel electrophoresis.

Supplier: EDVOTEK

ABO and Rh typing of blood left at the scene of a crime can help to narrow down a list of suspects. In this experiment, students use agglutination to identify the blood group of unknown blood samples as a step to identify a criminal.

Supplier: EDVOTEK

DNA obtained from a single hair left behind at a crime scene can be used to identify a criminal. In this experiment, students will compare simulated crime scene DNA with that of two suspects.

Supplier: EDVOTEK

Evidence left behind at a crime scene can identify a potential culprit. Even in this age of DNA, fingerprints and blood stains are still important in helping to identify a criminal. In this experiment, students solve a crime by dusting for fingerprints and use fluorescent dust to search for and identify trace amounts of blood.

Supplier: G-Biosciences

Dot blotting is a simple technique to identify a known protein in a biological sample. The ease and simplicity of the technique makes dot blotting an ideal diagnostic tool.

Supplier: EDVOTEK

Basic concepts of DNA fingerprinting are featured in this Ready-to-Load™ kit by comparing crime scene DNA with suspect DNAs. Fingerprint patterns are separated by agarose gel electrophoresis and the students determine who may have commited the crime.

Supplier: EDVOTEK

Ready-to-Load™ kit for teaching DNA fingerprinting and to show how DNA evidence is used in modern forensics. This experiment allows for varied results depending on the selection of DNA fingerprinting patterns.

Supplier: EDVOTEK

In this experiment, students see a blue colour change in transformed cells due to the switching on of a gene. The pGAL plasmid gives a blue colour dye due to the production of the β-galactosidase protein by the lacZ gene. IPTG is not required in this experiment since pGAL contains the complete lacZ gene.

Supplier: EDVOTEK

This experiment presents a bioterrorism scenario, with students examining a simulated DNA fingerprinting test for the detection of smallpox.

Supplier: EDVOTEK

Kit allows students to construct DNA models and then extract DNA from onions, strawberries or bananas.

Supplier: EDVOTEK

Experiment using fluorescence and electrophoresis to solve the mystery of the invisible bands.

Supplier: EDVOTEK

This Ready-to-Load™ kit shows how a restriction enzyme can be used to screen DNA for Sickle Cell Anaemia.

Supplier: EDVOTEK

Genetic testing can be used to identify people with a genetic condition which caused them to have an elevated level of cholesterol and which can be fatal. Students can see how genetic testing is carried out and learn about DNA electrophoresis.

Supplier: EDVOTEK

In this PCR experiment, students make billions of copies of a small amount of DNA in only 90 minutes. They just need to mix template DNA and primers with PCR beads that contain all of the other components required to carry out a PCR reaction. Students see the increasing amounts of DNA for themselves, taking samples every few cycles and analysing them on a DNA gel.

Supplier: EDVOTEK

In DNA fingerprinting, variable number tandem repeats (VNTR) are used to identify individuals. Using this kit, students type themselves at the D1S80 locus on chromosome 1. This region contains between 14 and 40 copies of a 16 base pair repeat.

Supplier: EDVOTEK

Learn the basics of DNA sequencing and sequence homology with this lab activity.

Supplier: EDVOTEK

For learning about the diversity of proteins by studying the electrophoretic profiles of various sources. Students separate proteins from bacterial, plant, serum, and milk proteins alongside a standard protein marker.

Supplier: EDVOTEK

This experiment introduces students to the principles of antigen-antibody interactions by using the Ouchterlony procedure. Antibodies and antigens form complexes that precipitate, making it possible to assay antibody-antigen systems. The binding interaction results in the formation of a white precipitate after diffusion in agarose.

Supplier: EDVOTEK

An HIV test detects HIV infection indirectly using an ELISA test against HIV antibodies in the blood. The test works by taking antibodies from the patient's blood and adding them to a microtitre plate coated with HIV antigen. If HIV antibodies are present, they will bind to the antigens on the plate. In this experiment, students perform an ELISA text by coating microtitre plate wells with simulated HIV antigen and then test simulated donor serum for anti-HIV antibodies.

Supplier: G-Biosciences

This DNA fingerprinting kit allows students to carry out their own criminal investigation by comparing DNA samples collected from suspects to DNA collected at a pseudo-crime scene.

Supplier: EDVOTEK

Students can solve a crime using PCR. Plasmid DNA is provided that, when amplified by PCR, provides products that represent individual DNA profiles.

Supplier: EDVOTEK

Coronary heart disease and stroke are major causes of death in the Western world. Elevated blood cholesterol levels are a serious risk factor in both conditions. The genetic disease familial hypersholesterolemia (FH) causes an increase in blood levels of the bad form of cholesterol, low density lipoprotein (LDL). In untreated patients with the mutant FH gene, the condition can cause premature death. This experiment introduces the colorimetric enzymatic reaction which is the basis of the clinical cholesterol test. In addition, using agarose gel electrophoresis, students can analyse a simulated genetic screening for a disease.

Supplier: EDVOTEK

Most molecules have a net charge within a pH range of 2 to 10. When the pH is altered, the net charge on molecules can change drastically. In this experiment, a mixture of 2 chemicals is absorbed onto a solid support ion-exchange column and separated during elution under conditions that influence their net charge.

Supplier: EDVOTEK

This kit is for teaching students how people's physical traits are a reflection of their genes. In this simulation, students use electrophoresis to separate dyes which represent genetic traits.