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Southern blotting is an important technique used widely in clinical genetics and research. By transferring DNA from an agarose gel onto a membrane the method allows the precise analysis and identification of DNA bands on a gel. Using this kit, students use Southern blotting to find a point mutation in the haemoglobin gene indicating Sickle Cell Anaemia.

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Microarray technology allows scientists to screen large numbers of samples in one assay. This technology allows high-throughput screening of small samples and allows comparisons to be made between healthy and unhealthy tissues. In this experiment, students apply simulated DNA and RNA samples to a membrane to screen for positive and negative samples.

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Isolation of high molecular weight chromosomal DNA is the first step in molecular cloning since it is the source of genes in cells. This experiment provides DNA extraction LyphoCells and reagents for isolating chromosomal DNA from E. coli. After spooling from solution, the DNA can be dissolved and analysed by agarose gel electrophoresis as an optional lab extension activity.

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This lab activity shows students what real chromosomal DNA looks like and allows them to explore the procedures involved in DNA extraction.

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Basic concepts of DNA fingerprinting are featured in this Ready-to-Load™ kit by comparing crime scene DNA with suspect DNAs. Fingerprint patterns are separated by agarose gel electrophoresis and the students determine who may have commited the crime.

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This experiment introduces a simple method to determine protein subunit molecular weights using horizontal electrophoresis. As the protein standards and 'unknowns' are prestained, the separation of proteins can be observed during electrophoresis. Included in the experiment is protein-grade agarose, which provides an alternative to the use of polyacrylamide gels.

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Kit for teaching students how to extract and precipitate their own DNA. Students can transfer their DNA to a tube that can be used as a pendant on a necklace.

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In this experiment, students purify the restriction enzyme, Eco RI. This procedure utilised an ion exchange chromatograph step for Eco RI purification. Column fractions are assayed for the enzyme using Lambda DNA and digestion products are identified by gel electrophoresis. Fractions that contain Eco RI are identified and pooled. The total and specific activities are calculated. Recommended for advanced courses.

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Bovine spongiform encephalophathy (BSE), also known as Mad Cow Disease, is a neurodegenerative, fatal condition in cattle. Consuming BSE-infected beef is believed to be the cause of a similar condition in humans, Creutzfeldt-Jakob disease. In this experiment, students examine simulated PCR products from several feed mills, to determine any possible violations of the ban on including animal parts in cattle feed.

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The mitochondria are thought to have evolved from a symbiotic relationship between prokaryotic and eukaryotic cells. Mitochondria have their own DNA and are only inherited via the maternal line. In this experiment, students amplify two regions of their mitochondrial DNA.

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For learning about the diversity of proteins by studying the electrophoretic profiles of various sources. Students separate proteins from bacterial, plant, serum, and milk proteins alongside a standard protein marker.

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This Ready-to-Load™ kit shows how a restriction enzyme can be used to screen DNA for Sickle Cell Anaemia.

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Learn the basics of DNA sequencing and sequence homology with this lab activity.

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The second assay used to confirm a positive HIV ELISA result is the Western blot. Using this kit, students separate protein samples from hypothetical patients on agarose gels. The proteins are then transferred to a membrane and simulated HIV proteins are detected.

Supplier: EDVOTEK

In this kit, a set of multi-coloured links demonstate a variety of molecular biology simulations. Students learn about digesting DNA with restriction enzymes, cloning genes in plasmids, protein structure, and more.

Supplier: EDVOTEK

In this PCR experiment, students make billions of copies of a small amount of DNA in only 90 minutes. They just need to mix template DNA and primers with PCR beads that contain all of the other components required to carry out a PCR reaction. Students see the increasing amounts of DNA for themselves, taking samples every few cycles and analysing them on a DNA gel.