Sekventeringsreagenser
Sekventeringsreagenser letter processerne, når den præcise rækkefølge af nukleotider i DNA-strenge skal bestemmes, ved at sortere fragmenterne efter størrelse. Enzymerne er optimeret til at arbejde sammen for effektiv fjernelse af ikke-inkorporerede primere og nukleotider. Disse reagenser giver fremragende sekventeringsresultater fra vanskelige skabeloner, såsom dem med højt GC-indhold og sekundære strukturer. Uundværlige i både grundlæggende og anvendt forskning gør sekventeringsreagenser det muligt for forskere at opdage genotyper og fænotyper hos prøver.
Fragment selection kits, Axygen® AxyPrep™ FragmentSelect-I Kit
Supplier: Corning
The AxyPrep™ FragmentSelect Kits utilise a paramagnetic bead technology for quick high throughput optimised DNA size selection suitable for various next generation sequencing platforms. The AxyPrep™ FragmentSelect-I kit is optimised for Illumina next generation sequencing platforms and Life Technologies (SOLiD™) fragment size selection needs simplifying the library construction workflow.
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ddCTP [2',3'-Dideoxycytidine-5'-triphosphate] *10 mM in ddH₂O* (1 µmole)
Supplier: AAT Bioquest
Sanger sequencing, also known as the chain termination method, is a technique for DNA sequencing based upon the selective incorporation of chain-terminating dideoxynucleotides (ddNTPs) by DNA polymerase.
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ddATP [2',3'-Dideoxyadenosine-5'-triphosphate] *10 mM in ddH₂O* (1 µmole)
Supplier: AAT Bioquest
Sanger sequencing, also known as the chain termination method, is a technique for DNA sequencing based upon the selective incorporation of chain-terminating dideoxynucleotides (ddNTPs) by DNA polymerase.
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ddGTP [2',3'-Dideoxyguanosine-5'-triphosphate] *10 mM in ddH₂O* (1 µmole)
Supplier: AAT Bioquest
Sanger sequencing, also known as the chain termination method, is a technique for DNA sequencing based upon the selective incorporation of chain-terminating dideoxynucleotides (ddNTPs) by DNA polymerase.
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MagaDye™ 561 ddATP
Supplier: AAT Bioquest
Sanger sequencing, also known as the chain termination method, is a technique for DNA sequencing based upon the selective incorporation of chain-terminating dideoxynucleotides (ddNTPs) by DNA polymerase.
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MagaDye™ 535 ddGTP
Supplier: AAT Bioquest
Sanger sequencing, also known as the chain termination method, is a technique for DNA sequencing based upon the selective incorporation of chain-terminating dideoxynucleotides (ddNTPs) by DNA polymerase.
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MagaDye™ 613 ddCTP
Supplier: AAT Bioquest
Sanger sequencing, also known as the chain termination method, is a technique for DNA sequencing based upon the selective incorporation of chain-terminating dideoxynucleotides (ddNTPs) by DNA polymerase.
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MagaDye™ 588 ddTTP
Supplier: AAT Bioquest
Sanger sequencing, also known as the chain termination method, is a technique for DNA sequencing based upon the selective incorporation of chain-terminating dideoxynucleotides (ddNTPs) by DNA polymerase.
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2-Aminoethoxypropargyl ddCTP (1 µmole)
Supplier: AAT Bioquest
Sanger method is one of the most reliable and earliest DNA sequencing methods.
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2-Aminoethoxypropargyl ddTTP (1 µmole)
Supplier: AAT Bioquest
Sanger method is one of the most reliable and earliest DNA sequencing methods.
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2-Aminoethoxypropargyl ddATP (1 µmole)
Supplier: AAT Bioquest
Sanger method is one of the most reliable and earliest DNA sequencing methods.
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2-Aminoethoxypropargyl ddGTP (1 µmole)
Supplier: AAT Bioquest
Sanger method is one of the most reliable and earliest DNA sequencing methods.
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ddTTP [2',3'-Dideoxythymidine-5'-triphosphate] *10 mM in ddH₂O* (1 µmole)
Supplier: AAT Bioquest
Sanger sequencing, also known as the chain termination method, is a technique for DNA sequencing based upon the selective incorporation of chain-terminating dideoxynucleotides (ddNTPs) by DNA polymerase.
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Arginine-C™, sequencing grade
Supplier: G-Biosciences
SG-Arginine-C™ endopeptidase (Clostripain, from C. histolyticum) specifically hydrolyses the carboxy peptide bond of arginine. SG-Arginine-C™ has been modified chemically by a propriety process to render the enzyme resistant to autolysis and stabilise enzymatic activity. In addition, as a sulfhydryl enzyme, SG-Arginine-C™ is susceptible to inactivation by oxidation and as a result requires reducing agents for protection. The enzyme also requires calcium ion for maximal activity. A special reconstitution buffer is supplied, which contains reducing agents and activators to maintain enzyme activity.
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Chymotrypsin™, sequencing grade
Supplier: G-Biosciences
SG-Chymotrypsin™ is a serine endopeptidase, which predominantly cleaves peptide bonds on the carboxy side of tyrosine, phenylalanine and tryptophan. In addition, chymotrypsin has a low catalytic activity against the carboxy side of leucine, methionine, alanine, aspartic and glutamic acids. It is therefore recommended to always use the shortest digestion time possible.
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Lysine-C™, sequencing grade
Supplier: G-Biosciences
SG-Lysine-C™ endopeptidase, from Lysobacter enzymogenes, is a serine protease highly specific in cleaving peptide bonds at the carboxy side of lysine. Highly purified preparations of SG-Lysine-C™ are chemically modified making the enzyme resistant to autolysis and stabilising its enzymatic activity.
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