buffers

Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.

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Buffer, SSC solution 20X concentrate (saline-sodium citrate), Sigma-Aldrich®

Supplier: Merck

Hybridisation Buffer, SSC buffer solution 20X concentrate (saline-sodium citrate)

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Tris Hydrochloride

Supplier: Merck Millipore (Calbiochem‎)

Use of tetraethylammonium (TEA) and Tris loading for blocking TRPM7 channels in intact cells.: This study highlights the application of TRIS Hydrochloride in blocking TRPM7 channels, which is significant for cell signaling and ion channel regulation in pharmacological research (Holderby & Kozak, 2024).

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Buffer solutions

Supplier: REAGECON

Buffer solution, pH value: 4,00, ±0,01, Reference temperature: 25 °C, Plastic bottle

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Phosphate buffered saline, 10X, for Western blots and IP, Sigma-aldrich®

Supplier: Merck

Phosphate buffered saline is routinely used as a wash buffer in Western blot and immunoprecipitation procedures, as well as during sample preparation of cell lysates for use in general molecular biology applications.

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Protein extraction reagent, BugBuster® HT, Millipore®

Supplier: Merck Millipore (Novagen)

BugBuster® HT Protein Extraction Reagent combines BugBuster® Protein Extraction Reagent and Benzonase® Nuclease in one convenient reagent. BugBuster® HT eliminates common bioprocessing problems resulting from traditional cell lysis procedures. Soluble proteins are gently extracted from E. coli without exposure to heat or oxidative damage and viscosity is eliminated by nucleic acid digestion in a single step. The resulting protein extract can easily be fractionated by conventional purification techniques. BugBuster HT is ideally suited for application in high throughput protein purifications.

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Buffer, TBE solution 10X concentrate (TRIS-borate-EDTA buffer), Sigma-Aldrich®

Supplier: Merck

TBE (Tris/Borate/EDTA) Buffer is commonly used in nucleic acid electrophoresis. This solution is effective under slightly basic conditions, which keeps DNA deprotonated, water-soluble, and protected from degradation. This concentrate can be easily diluted to 1X or 0,5X before use (with molecular biology grade water).

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pH buffer solutions

Supplier: Mettler - Toledo

Technical buffer solution, 6×250 ml, pH value: 4,64, Colourless, 250 ml

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Buffer, TRIS solution pH 7 (1 mol/L), Trizma®, Sigma-Aldrich®

Supplier: Merck

Cloning Buffer, TRIS buffer solution pH 7 (1 mol/l)

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Buffer, TRIS solution pH 7,5 (2 mol/L), Sigma-Aldrich®

Supplier: Merck

Cloning Buffer, TRIS buffer solution pH 7,5 (2 mol/l)

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pH buffer solutions, colour coded

Supplier: REAGECON

Colour coded pH buffer solutions for the calibration of pH meters.

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pH buffer solutions

Supplier: Hach

pH buffer standard solution with certificate of analysis, 4×250 ml, pH value: 7,00, Plastic bottle

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Glycine buffer, Sigma-Aldrich®

Supplier: Merck

For use in the enzymatic determination of lactate using lactic dehydrogenase.

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Triethylammonium bicarbonate buffers, for ion-exchange chromatography, Sigma-Aldrich®

Supplier: Merck

Buffer for use in ion-exchange chromatography and electrophoresis.

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Hank's balanced salt solution, modified, MP Biomedicals

Supplier: MP Biomedicals

Cell culture buffer, Hank's balanced salt solution, modified (1X)

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Phosphate buffered saline (PBS), tablet, Sigma-Aldrich®

Supplier: Merck

Phosphate buffered saline (PBS) is a buffer solution used in biological research. It is a water based salt solution containing sodium phosphate, sodium chloride and, in some formulations, it contains potassium chloride and potassium phosphate.