buffers

Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.

Buffer, TRIS-glycine-SDS solution 10X concentrate (TGS buffer), Sigma-Aldrich®

Supplier: Merck

Running Buffer, TRIS-glycine-SDS buffer solution 10X concentrate (TGS buffer)

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Buffer, PBS solution (phosphate buffered saline) pH 7,4, Sigma-Aldrich®

Supplier: Merck

Washing buffer, PBS buffer solution (phosphate buffered saline) pH 7,4

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VWR® Buffered EDTA

Supplier: KLINIPATH

Buffered EDTA for decalcifying fluids.

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Protexidase™, peroxidase protection buffer, Immuno™, MP Biomedicals

Supplier: MP Biomedicals

A peroxidase protective buffer which stabilises HRP conjugates. It has 500 ml of PBS, pH 7,2, containing 0,5% BSA, antimicrobial preservatives and proprietary stabilising compounds.

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Buffer, PBS solution 10X concentrate (phosphate buffered saline), Sigma-Aldrich®

Supplier: Merck

Washing buffer, PBS buffer solution 10X concentrate (phosphate buffered saline)

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Buffer, TRIS-glycine solution 10X concentrate (TG buffer), Sigma-Aldrich®

Supplier: Merck

Electrophoresis, Transfer buffer, TRIS-glycine buffer solution 10X concentrate (TG buffer)

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Buffer, TRIS-glycine solution 10X concentrate (TG buffer), Sigma-Aldrich®

Supplier: Merck

Electrophoresis, Transfer buffer, TRIS-glycine buffer solution 10X concentrate (TG buffer)

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EDTA disodium salt solution, 0,2% in phosphate buffered saline, MP Biomedicals

Supplier: MP Biomedicals

EDTA disodium salt, (2%) solution, in phosphate buffered saline without calcium and magnesium. Used as a gentle non-enzymatic cell dissociation reagent.

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10X Phosphate Buffered Saline (PBS), Sterile, Dulbecco's Formula, without Magnesium and Calcium

Supplier: MP Biomedicals

Role of a balanced salt solution in cell culture is multifaceted and can be divided into four principal functions: 1. Serves as an irrigating, transporting and diluting fluid while maintaining intra- and extracellular osmotic balance; 2. Provides cells with water and certain bulk inorganic ions essential for normal cell metabolism; 3. Combined with a carbohydrate, such as glucose, provides the principal energy source for cell metabolism; 4. Provides a buffering system to maintain the medium within the physiological pH range (7.2 - 7.6)