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Quinizarin green 95% (dye content), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Solvent Green 3, also known as Quinizarine Green SS (CI 61565) is an anthraquinone derivative. It is a green dye that imparts its colour to materials. Solvent Green 3 is made by condensing either leucoquinizarin or a mixture of leucoquinizarin and quinizarin with two moles of p-toluidine in the presence of boric acid.

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Crystal violet solution

Crystal violet solution

Supplier: VWR Chemicals

Primary stain for Gram staining

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Bathophenanthrolinedisulphonic acid disodium salt hydrate ≥95%, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Bathophenanthrolinedisulfonic acid disodium salt hydrate has been used to measure iron to determine the solubility of iron in the biological sample. It has been used as an iron chelator to grow plants without Fe-EDTA (ethylenediaminetetraacetic acid).

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Thymol blue sodium salt in ACS Reagent grade water 95% (dye content), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Thymol blue sodium salt in ACS Reagent grade water 95% (dye content), Sigma-Aldrich®

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2-Hydroxy-1,4-naphthoquinone 97%, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

2-Hydroxy-1,4-naphthoquinone is one among the forensic reagents used for fingerprint detection. 2-Hydroxy-1,4-naphthoquinone, produced from Lawsonia inermis, is an orange dye. It belongs to the class of naphthoquinone dyes. 2-Hydroxy-1,4-naphthoquinone is used to dye hair and to color textiles. It has antibacterial, antifungal, anti-inflammatory, antiviral and antineoplastic properties. 2-Hydroxy-1,4-naphthoquinone inhibits tumor cell growth. It stimulates the production of reactive oxygen species (ROS).

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Carbol fuchsin solution, AFB-color stain for bacteriology, Sigma-Aldrich®

Carbol fuchsin solution, AFB-color stain for bacteriology, Sigma-Aldrich®

Supplier: Merck

Tb-color carbol fuchsin solution is used to detect mycobacteria (AFB cold staining)

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trans-4-Hydroxycinnamic acid ≥98% (by HPLC), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

p-Coumaric acid has been used as a component of chemiluminescent substrate for protein detection in western blotting.

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Methyl blue, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Methyl blue, Sigma-Aldrich®

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Carbol fuchsin, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Carbol Fuchsin is a basic dye which forms a yellow-brown compound in presence of acid. It is mainly required for the staining of mycobacteria. The waxy walls of mycobacteria helps in retaining the dye within cells. It can also be used for the staining of bacterial spores.

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Basic fuchsin, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Basic fuchsin, Sigma-Aldrich®

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Eosin Y (yellowish), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Eosin Y (yellowish), Sigma-Aldrich®

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Basic fuchsin, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Basic fuchsin, Sigma-Aldrich®

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Azure A ≥70% (dye content), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Azure A is a basic cationic dye. The combination of azure A and silver staining is used for the quantification of glycosaminoglycans in agarose gels. It is also used for the staining of chromosomes in the nucleus. It is a main component of the azure B/polychromed methylene blue – eosin stains family, also referred to as the Romanowsky stain.

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Methyl blue microbiology stain (C.I. 42780)

Methyl blue microbiology stain (C.I. 42780)

Supplier: VWR Chemicals

Methyl blue microbiology stain (C.I. 42780)

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Direct red 80 25% (dye content), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Direct Red 80 (C.I. 35780) is an extremely hydrophilic, very large tetrakisazo hexasulfonated anionic dye that has a large, planar aromatic system.It is also known as Sirius red F3B, chlorantine fast red 7BLN or 5BRL, durazol brilliant red B, pontamine fast red 7BNL, and sirius fast red.

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Phloroglucinol, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Phloroglucinol (phlo) is a phenol derivative that shows a cytoprotective effect from oxidative damage by enhancing the activity of cellular catalase. It can also be used to prepare synthetic analogs of A-type proanthocyanidins (PACs) such as 2,8-dioxabicyclo nonane derivatives by reacting with the corresponding flavylium salts. Phlo can react with benzaldehyde derivatives to form phloroglucinol-based microporous polymeric organic frameworks (phlo-POF) with potential applications in ion exchange and gas adsorption. Phloroglucinol is an antispasmodic and a derivative of phenol.

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1-Methoxy-5-methylphenazinium methyl sulphate ≥95%, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

1-Methoxy-5-methylphenazinium methyl sulfate (MPMS) is a photochemically stable electron-transport mediator between NAD(P)H and tetrazolium dyes. The reduced form of MPMS converts tetrazolium salt to purple precipitate formazan. It is a very stable and non-enzymic electron carrier that mediates electron transfer by dehydrogenases in vitro to various electron acceptors such as tetrazolium dyes or the electrode of an enzymic electric cell.

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Haematoxylin solution (Mayer), Clin-Tech

Supplier: KLINIPATH

These ready to use staining solutions are used in histology and cytology staining.

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Wright's stain, certified by the Biological Stain Commission, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Wright stain, a modified romanowsky stain, is intended for differential staining of the cellular elements of blood. It comprises polychrome methylene blue and eosin B. Upon staining blood films with wright stain, the white blood cell nucleus and cytoplasm display characteristic blue or pink hues.

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Chromotrope 2R suitable for modified Gomori trichrome stain, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Chromotrope 2R has been used in the Gomori trichrome staining in osteochondral tissues. It has also been used for the staining of eosinophils in lung sections. Chromotrope 2R is used for the staining of microsporidia in body fluids and stool samples. It is used in modified Gomori′s trichrome staining. Chromotrope 2R has high affinity for phospholipids and is used for the staining of mitochondrial and endoplasmic reticulum membrane in histological samples.

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Malachite green oxalate, Technical Grade, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

The release of phosphate or pyrophosphate in an enzymatic reaction can be detected by malachite green and is measured spectrometrically at 660 nm.

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Neutral red, powder, Bioreagent cell culture reagent, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Neutral red (C.l. 50040, Cl Basic red 5) is a protonated diaminophenazine dye that has a small planar conjugated system.

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5-Fluorouracil ≥99% (by HPLC), powder, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

5-Fluorouracil has been used to induce apoptosis in cells. It has been used as a chemosensitising agent. A potent antitumor agent that affects pyrimidine synthesis by inhibiting thymidylate synthetase, thus depleting intracellular dTTP pools. It is metabolized to ribonucleotides and deoxyribonucleotides, which can be incorporated into RNA and DNA. Treatment of cells with 5-FU leads to an accumulation of cells in S-phase and has been shown to induce p53 dependent apoptosis.

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Pararosaniline hydrochloride ≥85% (by HPLC), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

This is the pure, principal component of mixtures commonly referred to as Basic Fuchsin.

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Congo red staining kit

Congo red staining kit

Supplier: Merck

Kit for the detection of amyloid acc. to Highman

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Wright's stain used as a blood stain, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Wright′s Stain solution is commercially available and is generally used in hematology. Its preparation involves heating methylene blue with sodium bicarbonate and combining the mixture with eosin. It is alkaline, hence, its concentration is continuously changing due to the progressive oxidizing action of the alkali. Therefore, Giemsa′s stain is added to preserve the azurophilic properties of polychrome dyes.

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Propidium iodide solution 1.0 mg/ml in water

Supplier: SIGMA ALDRICH MICROSCOPY

Membrane impermeant, phenanthridinium intercalator dye excited by mercury or xenon-arc lamps or argon-ion laser. Suitable for use in fluorescence microscopy, confocal laser scanning microscopy, flow cytometry, and fluorometry. Used as a nuclear counterstain.

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Fast green FCF, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Fast green FCF, Sigma-Aldrich®

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X-Gal (5-bromo-4-chloro-3-indolyl-ß-D-galactopyranoside) ≥99% (by enzymatic purity), Ultra Pure Grade

X-Gal (5-bromo-4-chloro-3-indolyl-ß-D-galactopyranoside) ≥99% (by enzymatic purity), Ultra Pure Grade

Supplier: VWR Chemicals

X-Gal (5-bromo-4-chloro-3-indolyl-ß-D-galactopyranoside) ≥99% (by enzymatic purity), Ultra Pure Grade

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Haematoxylin solution (Mayer), Clin-Tech

Supplier: KLINIPATH

Haemalum (Mayer’s)

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