Supplier: Tonbo Biosciences

The Hit3a antibody is specific for human CD3e, also known as CD3 epsilon, a 20 kDa subunit of the T cell receptor complex, along with CD3 gamma and CD3 delta. These integral membrane protein chains assemble with additional chains of the T cell receptor (TCR), as well as CD3 zeta chain, to form the T cell receptor – CD3 complex. Together with co-receptors CD4 or CD8, the complex serves to recognize antigens bound to MHC molecules on antigen-presenting cells. These interactions promote T cell receptor signaling (T cell activation), inducing cell proliferation, differentiation, production of cytokines or activation-induced cell death. CD3 is differentially expressed during thymocyte-to-T cell development and on all mature T cells.

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The RM4-5 antibody reacts with mouse CD4, a 55 kDa protein which acts as a co-receptor for the T cell receptor (TCR) in its interaction with MHC Class II molecules on antigen-presenting cells. The extracellular domain of CD4 binds to the beta2-domain of MHC Class II, while its cytoplasmic tail provides a binding site for the tyrosine kinase lck, facilitating the signaling cascade that initiates T cell activation. CD4 is typically expressed on thymocytes, certain mature T cell populations such as Th17 and T regulatory (Treg) cells, as well as on dendritic cells. The RM4-5 antibody is widely used as a phenotypic marker for CD4 expression. If used together, the RM4-5 antibody and an alternative antibody, Anti-Mouse CD4 clone GK1.5, will compete for binding, i.e. RM4-5 antibody is able to block GK1.5 antibody binding to cells. In contrast, RM4-5 antibody does not block the binding of Anti-Mouse CD4 clone RM4-4 to cells.

Supplier: Tonbo Biosciences

Goat anti-Rabbit IgG (H&L) - Affinity Pure, Alkaline Phosphatase Conjugate

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Donkey anti-Mouse IgG (H&L) - Affinity Pure, HRP Conjugate

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Goat anti-Rat IgG (H&L) - Affinity Pure, HRP Conjugate

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Donkey anti-Goat IgG (H&L) - Affinity Pure, HRP Conjugate, min x w/human, mouse, rabbit or rat IgG

Supplier: Tonbo Biosciences

Goat anti-Rabbit IgG (H&L) - Affinity Pure, min x w/ human, mouse or rat serum

Supplier: Tonbo Biosciences

Goat anti-Rabbit IgG (H&L) - Affinity Pure, HRP Conjugate, min x w/bovine, human, or mouse IgG

Supplier: Tonbo Biosciences

Rabbit anti-Human IgM (mu chain) - Affinity Pure

Supplier: Tonbo Biosciences

Goat anti-Rabbit IgG (H&L) - Affinity Pure, min x w/ bovine, goat, human, mouse or rat IgG or serum

Supplier: Tonbo Biosciences

Goat anti-Mouse IgG (H&L) - Affinity Pure, HRP Conjugate

Supplier: Tonbo Biosciences

Goat anti-Rabbit IgG (H&L) - Affinity Pure, FITC Conjugate, min x w/ bovine, goat, human, mouse or rat IgG or serum

Supplier: Tonbo Biosciences

Goat anti-Rabbit IgG (H&L) - Affinity Pure, Biotin Conjugate, min x w/ human, mouse or rat serum

Supplier: Tonbo Biosciences

Rabbit anti-Mouse IgG (H&L) - Affinity Pure, FITC Conjugate, min x w/human serum proteins

Supplier: Tonbo Biosciences

Goat anti-Rabbit IgG (H&L) - Affinity Pure, Biotin Conjugate, min x w/bovine, human, or mouse IgG

Supplier: Tonbo Biosciences

Goat anti-Rabbit IgG (H&L), F(ab')2 Fragment - Affinity Pure, FITC Conjugate, min x w/ bovine, human, or mouse IgG or serum proteins

Supplier: Tonbo Biosciences

Donkey anti-Mouse IgG (H&L) - Affinity Pure, HRP Conjugate, min x w/bovine, chicken, goat, guinea pig, hamster, horse, human, rabbit, rat or sheep IgG

Supplier: Tonbo Biosciences

Goat anti-Human IgM (mu chain) - Affinity Pure, min x w/human IgG; bovine, goat, mouse or rabbit serum proteins

Supplier: Tonbo Biosciences

Goat anti-Human IgA (alpha chain), F(ab')2 Fragment - Affinity Pure, FITC Conjugate

Supplier: Tonbo Biosciences

Goat anti-Human IgA (alpha chain), F(ab)'2 Fragment - Affinity Pure, Biotin Conjugate

Supplier: Tonbo Biosciences

Goat anti-Human IgM (mu chain) - Affinity Pure, min x w/human IgA + IgG

Supplier: Tonbo Biosciences

Goat anti-Human IgA (alpha chain) - Affinity Pure, Biotin Conjugate, min x w/bovine, mouse, and rabbit serum proteins

Supplier: Tonbo Biosciences

Goat anti-Mouse IgM (mu chain) - Affinity Pure, FITC Conjugate

Supplier: Tonbo Biosciences

Goat anti-Mouse IgM (mu chain) - Affinity Pure, FITC Conjugate, min x w/human IgG or serum proteins

Supplier: Tonbo Biosciences

Goat anti-Mouse IgG (H&L) - Affinity Pure

Supplier: Tonbo Biosciences

The M5/114.15.2 antibody reacts with mouse MHC Class II alloantigens I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek, as well as being cross-reactive with mouse cells of H-2p and H-2r haplotype. MHC Class II is widely expressed by mouse immune cells bearing these alloantigens, including T and B cells, monocytes, macrophages, and dendritic cells. The antibody does not react with the following alloantigens: I-Af, I-Ak, I-As, or NOD H-2g7. The M5/114.15.2 antibody may be used for analysis of mouse cells expressing MHC Class II alloantigens as described. Please note that the M5/114.15.2 clone may also be referred to as M5/114 in the literature.

Supplier: Tonbo Biosciences

The L243 antibody reacts with a member of the human MHC Class II antigens, HLA-DR. The HLA-DR antigen is expressed on B lymphocytes, activated T lymphocytes, activated NK cells, monocytes, macrophages, other antigen presenting cells and progenitor cells. The L243 antibody is specific to an epitope on the alpha subunit of the heterodimeric HLA-DR protein and binds a different epitope than the LN3 antibody clone. It does not cross-react with HLA-DP or HLA-DQ. This antibody is reported to be cross-reactive with non-human primates including Chimpanzee, Cynomolgus, Rhesus and Baboon. Please choose the appropriate format for each application.

Supplier: Tonbo Biosciences

The L243 antibody reacts with a member of the human MHC Class II antigens, HLA-DR. The HLA-DR antigen is expressed on B lymphocytes, activated T lymphocytes, activated NK cells, monocytes, macrophages, other antigen presenting cells and progenitor cells. The L243 antibody is specific to an epitope on the alpha subunit of the heterodimeric HLA-DR protein and binds a different epitope than the LN3 antibody clone. It does not cross-react with HLA-DP or HLA-DQ. This antibody is reported to be cross-reactive with non-human primates including Chimpanzee, Cynomolgus, Rhesus and Baboon. Please choose the appropriate format for each application.

Supplier: Tonbo Biosciences

The 2H7 antibody reacts with human CD20, expressed by pre-B cells, resting and activated B cells, follicular dendritic cells and a small subset of T cells, but not on plasma cells. CD20 is a 33-37 kDa transmembrane protein which is differentially phosphorylated in resting and active B cells. The CD20 molecule plays a role in regulating B-cell activation. The 2H7 antibody is reported to be cross-reactive with several non-human species including Baboon, Chimpanzee, Cynomolgus and Rhesus. Please choose the appropriate format for each application.

Supplier: Tonbo Biosciences

The RB6-8C5 antibody binds to mouse Ly-6G, commonly known as Gr-1, a member of the Ly-6 superfamily of GPI-anchored cell surface proteins with roles in cell signaling and cell adhesion. Gr-1 is differentially expressed during development and maturation of cells in the myeloid lineage and is expression at varying stages and levels on monocytes, macrophages, granulocytes, and peripheral neutrophils.