"Staining Solutions"
Differentiation Solution
Supplier: SIGMA ALDRICH MICROSCOPY
Differentiation solution comprises acidified ethanol or isopropanol that is used to decolourise hematoxylin stain. The duration of exposure to the solution determines the degree of decolourisation. Differentiation is a crucial step in hematoxylin-eosin staining to achieve the proper colour contrast necessary for visualisation, by selectively removing excess hematoxylin from the tissue section and leaving behind the desired staining intensity.
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Iron hematoxylin kit (Weigert's) stain for histology, Sigma-Aldrich®
Supplier: Merck
Nuclear staining in connective tissue staining methods
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Alcian blue solution used in nuclear staining, for histology, Sigma-Aldrich®
Supplier: Merck
Used to stain acidic mucosubstances
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Leishman's eosin-methylene blue modified solution stain for histology, Sigma-Aldrich®
Supplier: Merck
For staining of blood and bone marrow smears and clinical-cytological specimens
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Lugol's iodine solution, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Lugol′s solution is a ready-to-use solution that comprises 6% iodine in 4% potassium iodide. It is commonly used as a mordant for Gram staining in bacteriology. It is used for staining bacterial target structures for diagnostic purposes by fixing, staining, counterstaining, and mounting bacteriological specimen materials.
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Gram color staining kit, Sigma-Aldrich®
Supplier: Merck
Gram-colour staining kit, containing 500 ml each of crystal violet solution, Lugol's solution stabilised, Safranin solution and 2×500 ml decolourisation solution.
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Silver enhancer solution A, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Silver enhancer solution A has been used for silver staining.
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Papanicolaou's solution EA 50, Q Path® for microscopy
Supplier: VWR Chemicals
Ready to use solution for Papanicolaou cytological staining.
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7-Aminoactinomycin D (7-AAD) solution used in nuclear staining
Supplier: Tonbo Biosciences
This nucleic acid dye can be used to exclude non viable cells from the analysis of flow cytometry data.
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May Grunwald's eosin-methylene blue modified solution stain for hematology, for cytology, Sigma-Aldrich®
Supplier: Merck
Used to stain blood, bone marrow smears, and clinical cytological specimens
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Haemalum (Mayer's), Sigma-Aldrich®
Supplier: Merck
In hematoxylin-eosin staining (H&E), the most widely used histological staining technique, nuclear staining is achieved using ready-to-use staining solutions, such as Mayer’s hemalum solution or Hematoxylin solution modified according to Gill III. These solutions do not need to be filtrated prior use and the dye is oxidized in a controlled manner, which renders the results robust and allows a brilliant differentiation of the stained structures. Counterstaining of i.e. for proteins, collagen, keratin, intercellular substances. can be performed with alcoholic or aqueous Eosin Y solution 0.5 %. It is registered as IVD and CE certified product and can be used for clinical diagnostics.
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Giemsa's stain, Technical Grade used as a blood stain, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Giemsa stain is a versatile differential stain that is widely used in hematology for blood and bone marrow specimens, clinical cytology specimens, bacteriology, histological biopsies, and tumor samples. Giemsa's staining solution is composed of methylene blue, azure, and eosin. It is a major histological stain due to its unique staining of chromatin, nuclear membranes, and cytoplasmic elements. The stain is frequently used in combination with other dye solutions: May-Grünwald’s solution for Pappenheim (MGG) and Wright-Giemsa.
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Foxp3/transcription factor staining buffer
Supplier: Tonbo Biosciences
Foxp3/transcription factor staining buffer contains specially formulated buffers and solutions for optimal resolution and low background in your analysis of nuclear antigens by flow cytometry. This complete kit contains three components for use in these staining protocols for detection of nuclear antigens such as Foxp3 and ROR gamma. Foxp3/transcription factor fix/perm concentrate (4x) 50 ml. A concentrated solution which, when diluted with Foxp3/transcription factor fix/perm diluent (1x) provides best results in protocols for intranuclear staining of transcription factors using fluorescently conjugated antibodies.
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Mucicarmine solution (Mayer) for microscopy, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Most mucins contain serveral glycoproteins and can therefore be detected by stains for carbohydrates. Best carmine stain is specifically used for glycogens. Mucicarmine is an empirical stain. When applied for fungal staining, it stains the spores and hyphae as pale to dark red.
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Methylene blue solution
Supplier: VWR Chemicals
Staining solutions used in histology and cytology for the counterstaining of the cell nuclei red. Classically in a Gram staining and endospore staining.
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Schiff reagent for histology, Sigma-Aldrich®
Supplier: Merck
Ready-to-use staining solution for human-medical cell diagnosis.
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