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Gram color staining kit for tissue, Sigma-Aldrich®

Gram color staining kit for tissue, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Tissues are stained for Gram-positive organisms with crystal violet, mordanted in iodine, decolorized, stained for Gram-negative organisms with safranin O and then counterstained with tartrazine. For delineating Gram-positive and Gram-negative organisms

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Hydroquinone

Hydroquinone

Supplier: SIGMA ALDRICH MICROSCOPY

Intended for use in the Silver Stain (Steiner-Steiner) kit. Hydroquinone, 0.5 g each, with excipients.

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Metanil yellow 70% (dye content), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Metanil yellow has been used for staining of leukocyte granules. It is also used as a counterstain in microscopic sections.

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Tetramethylrhodamine isothiocyanate Isomer R (6-TRITC isomer R), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Tetramethylrhodamine isothiocyanate Isomer R (TRITC) is the R isomer of tetramethylrhodamine isothiocyanate and is also known as 6-tetramethylrhodamine isothiocyanate. It is a strongly fluorescent compound soluble in ethanol, methanol, dimethylformamide, dimethylsulfoxide, and water. TRITC is a moderately-sized aminoxanthene dye carrying carboxylic acid and isothiocyanate substituents on the non-planar pendant phenyl ring. It forms a lipophilic cation in acidic pH and a hydrophilic zwitterion around neutral pH. The isothiocyanate group reacts with nucleophilic substituents (e.g., amino, hydroxyl, thiol) of biomolecules, providing the means of attaching a fluorescent label. TRITC also reacts with water and hydroxide ions and is unstable in aqueous solutions. TRITC is commercially available as a zwitterion or as a chloride.

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N-Benzylidenebenzenesulphonamide ≥97%, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

N-Benzylidenebenzenesulfonamide,97%

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Fast Blue RR Salt, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Fast blue RR salt has been used in the immunohistochemistry to detect the activity of alkaline phosphatase. It has been used in the procedure of connective tissue staining using picrosirius red.

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Methyl blue, powder or crystals, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Methyl Blue is a triaminotriphenylmethane dye, widely used in paper, textile and leather production.

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Safranine O ≥85% (dye content), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Safranin O is a cationic dye, which displays less significant metachromasia. It is used to detect any variations in articular disease. Safranin O binds specifically to polyanions. It detects the amount of proteoglycan present in cartilages. Safranin O is used as a marker for glycosaminoglycan depletion in osteoarthritis. Safranin is used as a counter stain in gram staining.

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Catechol violet indicator, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Pyrocatechol Violet (PV) is a sulfone phthalein dye. It chelates metal ions. The metal-PV complex is bluish-violet in colour.

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N-Benzylidenebenzylamine, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Indicator for organolithium assay.

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Poly-L-lysine hydrobromide

Supplier: SIGMA ALDRICH MICROSCOPY

Poly-L-lysine is a positively charged amino acid polymer with approximately one HBr per lysine residue. The hydrobromide allows the poly-L-lysine to be in a crystalline form soluble in water. A small amount of product may be found in the beta structure because the HBr interferes with hydrogen bonding between amino and either the carboxyl groups or N or O containing moieties.

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Light green SF yellowish, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Light green SF yellowish, Sigma-Aldrich®

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Victoria blue R 80% (dye content), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Victoria Blue R is a basic dye. It belongs to the class of diphenylnaphthylmethane dyes. Victoria Blue R has been employed in manufacture of lake pigments. It has lot of industrial applications, but it is rarely used as a biological stain.

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Aqueous mounting medium, Fluoroshield™ with 1,4-Diazabicyclo[2.2.2]octane, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Fluoroshield™ with 1,4-Diazabicyclo[2.2.2]octane is an aqueous mounting medium for preserving fluorescence of tissue and cell smears.

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Nuclear Fast Red, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Nuclear fast red dye has anthraquinonic nature and is used either alone or in combination with other dyes. It is mainly used for staining of nuclei.

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Procion® Red MX-5B, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Procion Red has been used in the visualization of osteocyte canalicular system. It has been used as an adsorbate in the biosorption by Bacillus subtilis.

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