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Eosin Y (yellowish) 5 wt. % in H₂O in aqueous solution, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Eosin Y, also known as Eosin yellowish is a widely used xanthene dye. It is a counterstain that is used to distinguish between various connective tissue fibers and matrices, and the cytoplasm of different types of cells. Eosin in combination with hematoxylin is useful to demonstrate the general histological architecture of a tissue.

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2,3,5-Triphenyltetrazolium chloride (TTC) ≥98% (by HPLC), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

2,3,5-Triphenyltetrazolium chloride (TTC) ≥98% (by HPLC), Sigma-Aldrich®

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Leuco crystal violet, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Leucocrystal Violet has been used to identify histamine-producing lactobacilli in cheese.

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Carminic acid ≥90% (by HPLC), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Carmine is a cochineal product usually furnished as an aluminum and calcium salt of carminic acid. Carminic acid is used for human-medical cell diagnosis and serves the purpose of the histological investigation of sample material of human origin. It is suitable for staining nuclei in histological sections. It is utilised in various staining methods including the procedure for detection of glycogen and mucous substances (Best′s carmine), for nuclear staining, or vital staining.

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Thymol blue sodium salt in ACS Reagent grade water 95% (dye content), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Thymol blue sodium salt in ACS Reagent grade water 95% (dye content), Sigma-Aldrich®

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Methylene Violet 3RAX 90% (dye content), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Methylene Violet 3RAX has been used as a reagent in a preparation of citrate methylene violet, which is used for staining yeast cells. It is also used as an internal standard in tandem mass spectrometry (MS/MS) for the quantification of methylene blue in blood sample.

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1-(Methylamino)anthraquinone 98%, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

1-(Methylamino)anthraquinone 98%, Sigma-Aldrich®

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Fluorescein diacetate cell viability stain, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Fluorescein diacetate (FDA) is a non-fluorescent molecule, which is hydrolysed to fluorescent fluorescein in live cells. Dead cells cannot accumulate or hydrolyse FDA. Fluorescein diacetate has been used for the differentiation of live cells from dead cells.

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bisBenzimide H 33258 trihydrochloride (Hoechst 33258) ≥98% (by HPLC, TLC), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

bisBenzimide H 33258 has been used for nuclear staining in cells. bisBenzimide H 33258 is useful for staining DNA, chromosomes and nuclei. bisBenzimide H 33258 may be used for fluorescence microscopy or flow cytometry.

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Tetrazolium blue chloride for colorimetric determination of reducing compounds, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Tetrazolium blue chloride (BTC) is a colorless tetrazolium compound that upon reduction forms a colored diformazan. The reactivity has been applied to the analysis of reducing compounds including the nanomolar detection of reducing sugars and carbidopa and methyldopa. BTC has been used for histological staining of mouse coronal brain sections and rat brain sections. In combination with 4-nitrocatechol, tetrazolium blue chloride forms a ternary complex with molybdenum(VI) for extraction and spectrophotometric determination of molybdenum. It has also been used to stain Francisella tularensis cells for tularemic microagglutination test.

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Ferrozine mono-sodium salt hydrate, BioXtra, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

3-(2-Pyridyl)-5,6-diphenyl-1,2,4-triazine-4',4"-disulfonic acid sodium salt is also known as ferrozine.

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Xylenol orange tetrasodium salt in ACS Reagent grade water, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Xylenol orange tetrasodium salt is a fluorochrome acid dye with complexing and chelating properties that make it useful as an indicator in the spectrophotometric detection and assay of metal ions such as calcium, cadmium, bismuth, zinc, lead, and mercury. Its tetrasodium salt especially is used as an indicator in metal titrations. It changes color from yellow to red between pH 6.4 to 10.4. It has six potentially anionic substituents, one being sulfonate, and is anionic under staining conditions and during most laboratory applications.

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Purpurin(1,2,4-trihydroxy-9,10-anthraquinone) 90% (dye content), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Purpurin (1, 2, 4-trihydroxyanthraquinone) is the key pigment present in the roots of Indian madder (Rubia cordifolia). This pigment produces colors, with distinctive heat and light resistant properties.

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Lucigenin chemiluminescence reagent, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Reagent used for the generation of chemiluminescence in the presence of a reductant and oxygen; it is employed in numerous chemiluminescent assays. Lucigenin is specific for superoxide anion radical.

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Coomassie® brilliant blue R-250 in methanol for SDS-PAGE, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Brilliant Blue R concentrate is a methanol based stain especially designed for staining protein in polyacrylamide but will also stain agarose gels. The stain contains methanol and acetic acid so gels do not require fixing prior to staining. Brilliant Blue R Concentrate has been used for protein band detection after nondenaturing gradient gel electrophoresis, isoelectric focussing and SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis). For detection of protein bands following electrophoresis.

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Acid Fuchsin calcium salt ≥60 % (dye content), certified by the Biological Stain Commission, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Acid Fuchsin calcium salt,certified by the Biological Stain Commission, Dye content ≥60 % (Acid Magenta; Acid Rubin; Fuchsin S, SS, SS, ST, or SIII; Rubin S)

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Oil red O 0.5% in isopropanol (IPA), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Oil Red O stains lipid materials and imparts red-orange color to the lipids. It also marks vacuoles, particularly observed in ALL-L3 (acute lymphoblastic leukemia) or Burkitt′s lymphoma/leukemia. Oil Red O staining is done on fresh samples, as alcohol fixation removes lipids.

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6-CFDA (6-Carboxyfluorescein diacetate, single isomer), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Used to differentiate viable cells from apoptotic cells. Live cells that are not apoptotic accumulate 6-carboxyfluorescein but do not accumulate annexin. Fluorescent wavelengths λex 492 nm; λemm 517 nm in 0.1 M Tris, pH 8.0.

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5-DTAF hydrochloride, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

5-([4,6-Dichlorotriazin-2-yl]amino)fluorescein hydrochloride has been used for labeling of yeast cells to study the adhesion of yeast cells to the digestive tract of sea bass (Dicentrarchus labrax) larvae and leopard grouper (Mycteroperca rosacea).

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p-Xylene-bis(N-pyridinium bromide) (DPX) ≥95% (by HPLC), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

p-Xylene-bis(N-pyridinium bromide). ≥95% (TLC) (DPX)

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3,3'-Di-n-hexyloxacarbocyanine iodide [DiOC6(3) iodide] 98%

Supplier: SIGMA ALDRICH MICROSCOPY

3,3′-dihexyloxacarbocyanine iodide (DiOC6) is a supravital lipophilic fluorochrome. This cationic dye is employed to stain mitochondria and endoplasmic reticulum in animal and plant cells. In addition, it acts as a very rapid, consistent and highly selective fluorochrome for endophytic ascomycetes in plant roots and liverworts. DiOC6 also facilitates the detection of different mitochondrial changes happening during early apoptosis.

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Naphthol yellow S hydrate for microscopy, for the precipitation of amino acids and peptides, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Naphthol yellow S has been used as a staining agent in fixed human embryonic stem cell-derived cardiomyocytes.

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Haematoxylin, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Gill No. 1 formulation is used as a progressive cytology stain. Used with hematoxylin and eosin staining.

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Acid red 27 ≥85-95% (dye content), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Amaranth has been used as a dye for the microscopic visualization of crop contraction in blowflies. It has been used in the electrophoresis gel to track the migration front of the electrode buffer.

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Evans blue ≥75% (dye content), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Evans blue has been used to check cell viability. It has been used to study BBB permeability.

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3-Ethoxy-4-methoxybenzaldehyde ≥99%, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

3-Ethoxy-4-methoxybenzaldehyde is a derivative of vanillin. 3-Ethoxy-4-methoxybenzaldehyde may be used to synthesise Compound of Designation red 10 binding (CDr10b) to investigate its role in neuroinflammatory diseases.

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Hartman’s fixative histological tissue fixative, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Hartman′s fixative, which is also known as Davidson′s Fixative, contains acetic acid, alcohol, and formalin. It is widely used for the preservation of eye, bone marrow, breast, testis, and other tissues for histologic examination

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Thionin(acetate), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Thionin(acetate), Sigma-Aldrich®

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Keratin azure, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Suitable for use as a protease substrate.

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Bathophenanthrolinedisulphonic acid disodium salt hydrate ≥95%, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Bathophenanthrolinedisulfonic acid disodium salt hydrate has been used to measure iron to determine the solubility of iron in the biological sample. It has been used as an iron chelator to grow plants without Fe-EDTA (ethylenediaminetetraacetic acid).

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