118 Results for: "KAPA BIOSYSTEMS"

Supplier: KAPA BIOSYSTEMS

Accurate quantification of Next Generation Sequencing (NGS) libraries is crucial for reliable clonal amplification via either emulsion or bridge PCR. Underestimation results in non-clonality, while overestimation leads to inefficiency.

Supplier: KAPA BIOSYSTEMS

The KAPA™ Stranded RNA-Seq Kit with RiboErase offers a high quality, comprehensive solution for transcriptome sequencing. By utilising a targeted enzymatic method for depletion, the workflow enables superior reduction of ribosomal RNA (rRNA) and a more complete representation of the transcriptome, including precursor mRNAs and non-coding RNA (ncRNA). Kits also contain KAPAHiFi™ for high-efficiency and low-bias library amplification, and include a streamlined, 'with-bead' protocol.

Supplier: KAPA BIOSYSTEMS

KAPAHiFi™ is a second generation proofreading DNA polymerase engineered for extreme fidelity and robustness. Kit contains KAPAHiFi™ or KAPAHiFi™ Hot Start enzyme (1 unit/µl), dNTP Mix (40 mM), magnesium chloride (25 mM), Reaction Buffer GC (5X), and Reaction Buffer Fidelity (5X).

Supplier: KAPA BIOSYSTEMS

The KAPA™ T4 DNA Ligase catalyses the formation of a phosphodiester bond between 5' phosphate and 3' hydroxyl termini in duplex DNA or RNA. This enzyme will join blunt and cohesive end termini in 1 hour at room temperature as well as repair single stranded nicks in duplex DNA, RNA or DNA/RNA hybrids.

Supplier: KAPA BIOSYSTEMS

Ready to use 2X mix containing all components for PCR, except primers and template. The mix contains KAPAHiFi™ Hot Start DNA Polymerase in a proprietary reaction buffer, dNTPs (0,3 mM of each dNTP at 1X), MgCl₂ (2,5 mM at 1X) and stabilisers.

Supplier: KAPA BIOSYSTEMS

A second generation proofreading polymerase is used for the amplification of Next Generation Sequencing (NGS) libraries. KAPAHiFi™ is an innovative antibody-based Hot Start DNA polymerase engineered via a process of molecular evolution for highest fidelity and low GC bias.

Supplier: KAPA BIOSYSTEMS

The KAPA™ Library Preparation Kit provides all the enzymes and reaction buffers required for constructing Illumina libraries from fragmented dsDNA. Reaction buffers are supplied with all of the required reaction components except oligonucleotide adaptors or PCR primers. Similarly, a single enzyme mixture is provided for each step of the library construction process, reducing the number of pipetting steps.

Supplier: KAPA BIOSYSTEMS

The 2X KAPA2G™ Robust ReadyMix is recommended for all standard end-point PCR assays, even with GC-rich templates or Colony PCR. Amplicons generated with the KAPA2G Robust ReadyMix are suitable for routine downstream applications, including restriction enzyme digestion, cloning and sequencing.

Supplier: KAPA BIOSYSTEMS

KAPA™ SYBR® FAST master mixes contain a novel DNA polymerase engineered via a process of molecular evolution. The result is a unique enzyme, specifically evolved for qPCR using SYBR® Green I dye chemistry. The KAPA™ SYBR® DNA Polymerase has been engineered to perform optimally in stringent qPCR reaction conditions, exhibiting dramatic improvements in fluorescence, Ct values, linearity, and sensitivity. Applications: Gene expression analysis, microarray validation, low-copy detection, gene knockdown validation, absolute quantification of NGS libraries.

Supplier: KAPA BIOSYSTEMS

KAPA™ Probe Fast qPCR master mixes are designed for highly sensitive and accurate Real-Time PCR using sequence-specific fluorogenic probe chemistries, including hydrolysis probes (eg. TaqMan), FRET probes, and displacement probes (eg. Molecular Beacons).

Supplier: KAPA BIOSYSTEMS

KAPA™ Express Extract is a thermostable protease and buffer system that allows for the extraction of PCR-ready DNA from various tissue types in as little as 15 minutes. The KAPA™ Express Extract system has been designed for optimal tissue lysis and DNA preservation. DNA extractions are conveniently performed in a single-tube, without the need for hazardous chemicals and multiple washing steps, thereby greatly reducing the risk of sample loss and contamination.

Supplier: KAPA BIOSYSTEMS

The KAPA™ Blood PCR MasterMix allows the amplification of DNA fragments directly from reactions containing 1 to 20% (v/v) whole human blood without pretreatment of blood samples or DNA isolation, significantly reducing contamination risk, turnaround time and cost of genetic testing.

Supplier: KAPA BIOSYSTEMS

Using the KAPA2G™ Fast PCR Kit up to 70% of total PCR time can be reduced with no requirement for specialist PCR consumables or instrumentation.

Supplier: KAPA BIOSYSTEMS

Amplification of plant-derived DNA is a challenging application due to the diversity of plant tissue types and the potent PCR inhibitors contained within the tissue. The KAPA3G Plant PCR Kit is optimised for direct PCR from crude plant samples and crude extraction methods.

Supplier: KAPA BIOSYSTEMS

KAPA™ primer prefix for ABI SOLiD

Supplier: KAPA BIOSYSTEMS

KAPA™ Mouse Genotyping for the rapid extraction and amplification of DNA from mouse tissue in less than 1 hour. The kit includes KAPA™ Express Extract, a novel thermostable protease and buffer system that allows the extraction of PCR-ready DNA from mouse tissue in as little as 15 minutes in a single-tube, and the KAPA2G Fast Hot Start Genotyping Mix with dye, containing a DNA polymerase engineered via a process of molecular evolution for high processivity and extreme speed.

Supplier: KAPA BIOSYSTEMS

Using the KAPA2G™ Fast Hot Start ReadyMix or the KAPA2G™ Fast ReadyMix with Dye up to 70% of total PCR time can be reduced with no requirement for specialist PCR consumables or instrumentation. A second-generation polymerase for the ultimate in speed and performance.

Supplier: KAPA BIOSYSTEMS

The KAPA™ Hyper Prep Kit is a versatile, streamlined solution for DNA library preparation for Illumina sequencing. The one-tube chemistry, optimally formulated, and evolved enzymes enable higher yields of adapter-ligated library and lower amplification bias. This translates to higher library diversity, lower duplication rates and more uniform coverage, particularly for FFPE and low-input samples.

Supplier: KAPA BIOSYSTEMS

KAPA™ Stranded RNA- and mRNA-Seq Kits offer a high quality solution for transcriptome sequencing on Illumina platforms. Through the use of high-quality reagents, including KAPAHiFi™ for low bias library amplification, and an optimised workflow, the kit demonstrates improved coverage of difficult transcripts, including those that are GC-rich and in low-abundance. KAPA RNA-Seq is offered in two versions:

Supplier: KAPA BIOSYSTEMS

KAPA Pure Beads offer a tunable and highly consistent solution for reaction purification and size selection in DNA and RNA next-generation sequencing library construction workflows.

Supplier: KAPA BIOSYSTEMS

The KAPA2G™ Robust PCR kit is recommended for all standard end-point PCR assays, even with GC-rich templates or Colony PCR. Amplicons generated with the KAPA2G Robust PCR kit are suitable for routine downstream applications, including restriction enzyme digestion, cloning and sequencing.

Supplier: KAPA BIOSYSTEMS

KAPA SYBR® FAST One-Step qRT-PCR Kits contain M-MuLV Reverse Transcriptase, RNase Inhibitor and a novel DNA Polymerase engineered via molecular evolution. The kit is optimised for rapid one-step, one-tube RNA quantification. KAPA SYBR® FAST One-Step qRT-PCR Kits are designed to perform optimally in stringent qRT-PCR reaction conditions, exhibiting dramatic improvements in sensitivity, specificity, reaction efficiency, and fluorescence.

Supplier: KAPA BIOSYSTEMS

KAPA™ HRM Fast is a convenient, ready to use 2X master mix designed for High Resolution Melt (HRM) analysis. The mix contains a novel DNA polymerase and buffer system, and EvaGreen® dye optimised for maximum discrimination between sequence variants. KAPA™ HRM Fast is ideally suited for: SNP genotyping, mutation discovery, screening for heterozygosity, DNA fingerprinting, DNA methylation analysis.

Supplier: KAPA BIOSYSTEMS

KAPA HYPERPLUS KIT AGILENT XT Fast KAPA HyperPlus Kit for SureSelectXT Fast Target Enrichment (96 Reaktionen) 1 * 96 Tests

Supplier: KAPA BIOSYSTEMS

KAPA SYBR FAST UNIVERSAL 10 ml für 1000 Reaktionen à 20 µl 1 * 10 ml

Supplier: KAPA BIOSYSTEMS

KAPA SYBR® FAST Universal 2X qPCR Master Mix (2 x 5 mL =10 mL) 1 * 1 KIT

Supplier: KAPA BIOSYSTEMS

KAPA PRIMER-PREMIX 129 BP (10x) KAPA Primer-Premix 129 bp (10x)Bestandteil des KAPA hgDNA Quantification and QC Kits für die Real-Ti me PCR-basierte Quantifizierung und Qualitätsüberprüfung von humaner genomischer DNA vor der Konstruktion einer NGS-B 1 * 200 µl

Supplier: KAPA BIOSYSTEMS

KAPA DNA STANDARD 0 für Illumina, 1 x 80 µl, 200 pM Der 452 bp KAPA DNA-Standard besteht aus einem linearen DNA-Fragment, das von den Illumina Primer-Bindestellen flankiert wird. Versand: Kühlakkus Lagerung: -20°C 1 * 80 µl

Supplier: KAPA BIOSYSTEMS

KAPA PROBE FORCE KAPA PROBE FORCE ist ein Mastermix für die sondenbasierte Real-Time PCR mit hoher Resistenz gegenüber Inhibitoren. Er macht die DNA-Aufreinigung überflüssig und ermöglicht kurze Protokolle. Der Mastermix enthält eine DNA-Polymerase d 1 * 10 ml

Supplier: KAPA BIOSYSTEMS

KAPA SYBR FAST ABI PRISM 10 ml für 1000 Reaktionen à 20 µl Ready-To-Use 2x qPCR Fast MasterMix mit speziell designter KAPA SYBR Fast DNA Polymerase zur Quantifizierung von DNA Templates mittels SYBR Green Assays. Für die folgenden Real-Time Cycler ge 1 * 10 ml