10503 Results for: "ENZO LIFE SCIENCES"

Supplier: Enzo Life Sciences

This kit is a complete assay system for measuring caspase-3 and caspase-3-like activity (DEVDase) in cellular extracts.

Supplier: Enzo Life Sciences

The PTP1B Tyrosine Phosphatase Drug Discovery Kit is a colorimetric, non-radioactive assay designed to measure the phosphatase activity of purified PTP1B. This 96-well assay is useful for screening inhibitors and modulators of PTP1B activity.

Supplier: Enzo Life Sciences

Chaperonin 10 (Cpn10 or Hsp10), is the ~10 kDa mammalian equivalent to GroES of E. coli. Chaperonins play fundamental roles in the folding, assembly, and translocation of other proteins.

Supplier: Enzo Life Sciences

Necrosis inhibitor

Supplier: Enzo Life Sciences

H2S donor

Supplier: Enzo Life Sciences

UCH inhibitor

Supplier: Enzo Life Sciences

The Hsp90 family of heat shock proteins represents one of the most abundantly expressed and highly conserved families of cellular chaperones whose expression can be upregulated under conditions of cellular stress, and includes cytoplasmic (Hsp90-alpha/beta), ER (grp94), and mitochondrial (TRAP1) localized members. Structurally, Hsp90 is characterized by an N-terminal ATP-binding domain, a medial substrate-binding domain, and a C-terminal dimerization motif. Hsp90 dimers function in cooperation with cochaperones (e.g. Hsp40, Hsp70, Hop, p23) to stabilize a multitude of client protein substrates, including steroid hormone receptors, protein kinases, and transcription factors. The essential binding and hydrolysis of ATP by Hsp90 is inhibited by ansamycin drugs (e.g. geldanamycin, 17-AAG) which occupy the N-terminal Hsp90 nucleotide-binding pocket. Many Hsp90 client proteins such as erbB2/Her-2, c-raf, bcr-abl, p53, and hTERT, are members of well characterized oncogenic pathways, making Hsp90 inhibitors useful anticancer agents.

Supplier: Enzo Life Sciences

The 70 kDa heat shock protein Hsp70 belongs to the Hsp70 family of highly-related protein isoforms ranging in size from 66 kDa to 78 kDa. Hsc70 shares close biochemical and biological ties to Hsp70, and also belongs to the Hsp70 family. These proteins include cognate members found within major intracellular compartments and highly inducible isoforms predominantly cytoplasmic or nuclear in distribution. Members of the Hsp70 family function as molecular chaperones involved in such cellular functions as protein folding, transport, maturation and degradation, operating in an ATP-dependent manner. The molecular chaperones of the Hsp70 family recognize and bind to nascent polypeptide chains or partially folded intermediates of proteins, preventing their aggregation and misfolding, and the binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein. Data demonstrates that with a ubiquitin-like domain at its amino terminus and its association with the 26S proteosome in HeLa cells, Bag-1 modulates the chaperone activity of Hsc70 and Hsp70. These findings reveal Bag-1's role as a physical link between the Hsc70/Hsp70 chaperone system and the proteasome. Experimental data also shows that the ATPase domain and the substrate binding domain of Hsd70 cooperate to form a co-chaperone-chaperone complex with the synaptic vesicle cysteine string protein (csp), essential for normal neurotransmitter release.

Supplier: Enzo Life Sciences

Produced in Sf21 cells.

Supplier: Enzo Life Sciences

Actin is one of the major proteins (~42 kDa) of the cytoskeleton. It regulates contractile potential in muscle tissues and helps to control the shape as well as the motility of non-muscle cells. Actin is expressed in all eukaryotic cells which makes it a useful loading control in Western blotting experiments. In higher eukaryotes, there are six isoforms of actin: Three alpha-actins (α-skeletal, α-cardiac, α-smooth muscle), one β-actin (β-non-muscle) and two γ-actins (γ-smooth muscle and γ-non-muscle). Actin isoforms show >90% overall sequence homology.

Supplier: Enzo Life Sciences

Immunohistochemistry (mouse/rabbit-HRP) detection reagent is a non-biotin one-step detection reagent.

Supplier: Enzo Life Sciences

The IHC PAP pen provides a hydrophobic barrier when a circle is drawn around a specimen on a glass slide.

Supplier: Enzo Life Sciences

Blue IHC chromogen (HRP) is intended to be used in conjunction with horseradish peroxidase (HRP)-based immunostaining or in situ hybridisation systems.

Supplier: Enzo Life Sciences

Spin trapping reagent

Supplier: Enzo Life Sciences

Host: Rabbit

Supplier: Enzo Life Sciences

Pannexin-1 belongs to a family of membrane proteins with homology to invertebrate gap junction proteins innexins. Pannexin-1 has been involved in the NALP (inflammasome)-mediated caspase-1 activation and IL-1β secretion.

Supplier: Enzo Life Sciences

Retinoid X receptor ligand.

Supplier: Enzo Life Sciences

Retinoic acid receptor ligand.

Supplier: Enzo Life Sciences

Isolated from Microcystis aeruginosa.

Supplier: Enzo Life Sciences

Host: Rabbit, Isotype: IgG

Supplier: Enzo Life Sciences

Host: Rabbit, Isotype: IgG

Supplier: Enzo Life Sciences

Antitumor antibiotic which inhibits cell cycle at the G(2)/M phase and induces apoptosis.

Supplier: Enzo Life Sciences

Antitumor antibiotic. Inhibits the growth of Gram-positive bacteria including MRSA.

Supplier: Enzo Life Sciences

Recommended Applications: ELISA, WB

Species reactivity: Human

Supplier: Enzo Life Sciences

A QuantiZyme™ Assay System. The Cathepsin S Drug Discovery Kit is a complete assay system designed to screen inhibitors of cathepsin S. Using a convenient microplate format, cathepsin S activity is monitored fluorimetrically using the substrate provided in the kit.

Supplier: Enzo Life Sciences

A QuantiZyme™ Assay System. The MMP-13 Colorimetric Drug Discovery Kit is a complete assay system designed to screen inhibitors of matrix metalloproteinase-13 (MMP-13, collagenase-3) using a thiopeptide as a chromogenic substrate.

Supplier: Enzo Life Sciences

A QuantiZyme™ Assay System. The MMP-14 Fluorometric (also known as fluorimetric) Drug Discovery Kit is a complete assay sytem designed to screen for inhibitors of matrix metalloproteinase-14 (MMP-14, membrane-type matrix metalloproteinase 1, MT1-MMP) using a quenched fluorogenic peptide: OmniMMP™ fluorogenic substrate Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH. Mca fluorescence is quenched by the Dpa group until cleavage by MMPs at the Gly-Leu bond separates the two moieties.

Supplier: Enzo Life Sciences

Topoisomerase I inhibitor

Supplier: Enzo Life Sciences

A QuantiZyme™ Assay System. The MMP-20 Colorimetric Drug Discovery Kit is a complete assay system designed to screen inhibitors of matrix metalloproteinase-20 (MMP-20, enamelysin) using a thiopeptide as a chromogenic substrate.

Supplier: Enzo Life Sciences

Cell permeable inhibitor of necrosis. Selectively blocks oxidative stress-induced necrotic cell death (~3 µm IM-54 prevented ~50% cell death in HL60 cells exposed to 100 µm H₂O₂). Does not protect against etoposide-induced apoptosis or display antioxidant properties. Does not affect S6K1 and PKC kinase activities at concentrations of 50 µm.