"EDVOTEK"
Single channel pipettes, fixed volume, MiniPipettes
Supplier: EDVOTEK
Micropipettes which use standard pipette tips. No need to calibrate and near impossible to measure the wrong volume.
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Experimentation kits, PCR-based Alu-Human DNA typing
Supplier: EDVOTEK
Students use primers for a 300 base pair Alu insertion in chromosome 16 (PV92) to determine their own genotype. They can then compare their class results with others around the world over the internet.
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Experimentation kits, blood typing
Supplier: EDVOTEK
ABO and Rh typing of blood left at the scene of a crime can help to narrow down a list of suspects. In this experiment, students use agglutination to identify the blood group of unknown blood samples as a step to identify a criminal.
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Experimentation kits, water quality testing I: chromogenic analysis of water bacteria contaminants
Supplier: EDVOTEK
Safe drinking water is vitally important to health. Both pathogenic and harmless bacteria can be found in the guts of mammals and birds. Testing water for every possible type of pathogenic bacteria is slow and costly. Thus, water is tested for a characteristic type of gut bacteria - the coliforms - including the familiar E. coli. Presence of coliforms is an indicator for faecal contamination. In this experiment, students test for coliforms in simulated contaminated water using colour and fluorescent reagents. They can use these same reagents to test water samples from the environment. As an extension activity, a Gram Stain test can be performed on the collected samples.
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Experimentation kits, determination of protein molecular weight (polyacrylamide-based)
Supplier: EDVOTEK
Using prestained LyphoProteins, subunit molecular weights are determined by analysis using denaturing SDS vertical polyacrylamide gel electrophoresis. Prestained proteins with unknown molecular weights are assigned molecular weights based on the relative mobility of prestained standard protein markers.
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Experimentation kits, analysis and comparision of mammalian cell types
Supplier: EDVOTEK
Observe the differences between mammalian cell types and how these cells funciton. Cells are fixed on microscope slides and students stain the cells on the slide to view morphological characteristics of the cell types. These cells are very safe for classroom use.
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Transformation with green fluorescent protein
Supplier: EDVOTEK
In this experiment, transformed cells take up a plasmid containing the Green Fluorescent Protein (GFP) gene. The GFP gene was isolated from the jellyfish Aequorea victoria. Transformed colonies expressing the GFP protein are visibly green in normal light but will fluoresce brightly when exposed to longwave UV light.
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Transformation of E. coli with pGAL™
Supplier: EDVOTEK
In this experiment, students see a blue colour change in transformed cells due to the switching on of a gene. The pGAL plasmid gives a blue colour dye due to the production of the β-galactosidase protein by the lacZ gene. IPTG is not required in this experiment since pGAL contains the complete lacZ gene.
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Micropipetting basics
Supplier: EDVOTEK
Kit for teaching students how to use a micropipette with ease and accuracy using multi-coloured dyes.
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Pipette stand for six micropipettes
Supplier: EDVOTEK
Stand, pipette, For: 6 pipettes
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UltraSpec-Agarose™, DNA electrophoresis grade
Supplier: EDVOTEK
Electrophoresis Gels, UltraSpec-Agarose™
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Horizontal Electrophoresis Apparatus, M6 and M12
Supplier: EDVOTEK
Capable of running one or two groups of student samples at the same time and producing excellent results in 30 to 40 minutes.
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Purification and size determination of green and blue fluorescent proteins
Supplier: EDVOTEK
When bacteria are used to make medicinally useful proteins by transformation, the protein of interest must be separated from all of the other cellular proteins. In this experiment, the unique fluorescent properties of GFP and BFP are used as an assay during their purification from an E. coli extract. The column fractions containing GFP or BFP are identified by fluorescence and then purified. As an optional activity, purified protein fractions can be separated by SDS polyacrylamide gel electrophoresis (SDS-PAGE) to estimate the purity and size of the GFP and BFP proteins.
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