2129 Results for: "Cytiva"

Supplier: Cytiva

Protein G Sepharose 4 Fast Flow has recombinant protein G immobilized by the cyanogen bromide (CNBr) method to Sepharose 4 Fast Flow.

Supplier: Cytiva

NHS-activated Sepharose™ 4 Fast Flow is composed of cross-linked 4% agarose beads, NHS activated for coupling amino-containing proteins and peptides in process-scale applications. NHS (N-hydroxysuccinimide) coupling forms a chemically stable amide bond with ligands containing primary amino groups. The resin provides a spacer arm to the coupled ligand and suitable for immobilising smaller protein and peptide ligands.

Supplier: Cytiva

TempliPhi™ Kits efficiently prepare micrograms of circular DNA from picogram input material. The DNA templates are prepared by rolling circle amplification (RCA) using bacteriophage Phi29 DNA polymerase. TempliPhi™ uses an isothermal method for the exponential amplification of circular DNA. Phi29 DNA polymerase is active at 30 °C, enabling amplification to be performed at this temperature without the need for thermal cycling. The TempliPhi™ protocol requires less than 20 minutes of hands-on time to amplify 96 samples from bacterial colonies.

Supplier: Cytiva

HiTrap Q FF is prepacked with Q Sepharose Fast Flow, and is a strong anion exchanger for small-scale protein purifications as well as screening of binding and elutions conditions.

Supplier: Cytiva

DEAE Sepharose™ Fast Flow is part of the Sepharose™ Fast Flow ion exchange platform. It is composed of cross-linked 6% agarose beads, with diethylaminoethyl (DEAE) weak anion exchange groups. Scale-up with DEAE Sepharose™ Fast Flow is straight forward.

Supplier: Cytiva

Superdex 200 Increase size exclusion chromatography columns are well suited for high-resolution analysis and small-scale purification of monoclonal antibodies (mAb) and other biomolecules with Mr~ 10000 to 600000.

Supplier: Cytiva

Polyetheretherketone tubing (PEEK) is intended for use with AKTA systems.

Supplier: Cytiva

ÄKTA start is an easy to use chromatography system that automates manual purification procedures using, for example, HiTrap columns. It offers an entry level chromatography solution for the preparative purification of proteins at lab scale.

Supplier: Cytiva

The 2-D Clean-Up Kit is designed to prepare samples for 2-D electrophoresis that otherwise produce poor 2-D spot-maps due to high conductivity, high levels of interfering substances, or low protein concentration. The reagents quantitatively precipitate proteins while leaving interfering substances, such as detergents, salts, lipids, phenolics, and nucleic acids, in solution.

Supplier: Cytiva

HisTrap FF Crude columns are high binding capacity IMAC columns for his-tagged protein purification when scalability is needed.

Supplier: Cytiva

HiTrap Heparin HP columns are designed for high-resolution purification of DNA-binding proteins, coagulation factors, and other plasma proteins.

Supplier: Cytiva

PD-10 Desalting Columns contain Sephadex G-25 resin for rapid buffer exchange, desalting, and removal of small contaminants (salts, dyes, radioactive labels) from samples using gravity flow or centrifugation.

Supplier: Cytiva

Immobiline™ drystrip cover fluid

Supplier: Cytiva

Highly species specific HRP-conjugated antibodies optimized for use with Amersham ECL Western Blotting Detection Reagents.

Supplier: Cytiva

On-line filter kit (20, 50, and 100 ml/min systems), PP, contains 10× filter, 2× support screen

Supplier: Cytiva

Percoll® PLUS is for preparation of cells, subcellular particles and larger viruses.

Supplier: Cytiva

Glutathione Sepharose™ 4B is designed for high capacity single-step purification of Glutathione S-Transferase (GST) tagged fusion proteins.

Supplier: Cytiva

Amersham™ Hybond™-N+ is a positively charged nylon membrane with a binding capacity for nucleic acids up to 600 µg/cm² recommended for use with radioactive or non radioactive chemiluminescence and chemifluorescence detection systems.

Supplier: Cytiva

Amersham™ Hybond™-XL is a positively charged nylon membrane which is designed to give optimum signal-to-noise ratios when used with radioactively labelled probes. Amersham™ Hybond™-XL has a greater concentration and a more even distribution of positive charge than other charged nylon membranes. It retains all the features of other nylon membranes, including high binding capacity up to 600 µg/cm², efficient binding of small fragments, effective probe removal and exceptional properties for reprobing.

Supplier: Cytiva

illustra™ MicroSpin™ S-400 HR columns designed for rapid purification of PCR products (>200 bp) from unincorporated primers (<32-mers) and nucleotides using spin-column chromatography.

Supplier: Cytiva

Amersham ECL Prime Blocking Reagent is blocking Amersham Protran NC and Amersham Hybond PVDF membranes used with Amersham ECL start, Amersham ECL, Amersham, ECL Prime, Amersham ECL Select and Amersham ECL Plex.

Supplier: Cytiva

The illustra GFX PCR DNA and Gel Band Purification kits are for the isolation and concentration of DNA fragments from PCR mixtures, DNA-containing agarose gel bands, enzyme-based DNA modifications, and restriction enzyme digests.

Supplier: Cytiva

XK empty columns are designed for liquid chromatography at low to medium pressure. The user friendly design ensures trouble-free operation with fittings for direct connection to high performance liquid chromatography systems. They are compatible with aqueous solutions and most organic solvents used in liquid chromatography of macromolecules. They are not resistant to acetone, ktones, chlorinated hydrocarbons, aliphatic esters and phenol. Should be avoided: >10% NaOH, >10% HCl, >5% acetic acid and strong mineral acids.

Supplier: Cytiva

illustra™ ExoProStar™ 1-STEP Kit uses illustra™ Exonuclease I and Alkaline Phosphatase for optimal removal of unincorporated primers and nucleotides. illustra™ ExoProStar™ improves digestion efficiency with no degradation of the target PCR product. The sample remains completely intact and ready for immediate use in manual or automated processes.

Supplier: Cytiva

Ficoll-Paque™ PREMIUM products are a range of sterile, ready to use density gradient media for the preparation of mononuclear cells.

Supplier: Cytiva

HisTrap™ FF columns are designed for simple, one-step purification and scaling up purification of histidine-tagged proteins. The columns are pre-packed with Ni Sepharose™ 6 Fast Flow.

Supplier: Cytiva

Ni Sepharose 6 Fast Flow is a BioProcess medium that combines the advantages of Ni₂₊ for purification of histidine tagged proteins, with the well industrially established properties of the Sepharose Fast Flow platform.

Supplier: Cytiva

NAP™ columns are disposable columns prepacked with Sephadex™ G-25 DNA grade and require only gravity to run.

Supplier: Cytiva

Highly species specific HRP-conjugated antibodies optimized for use with Amersham ECL Western Blotting Detection Reagents.

Supplier: Cytiva

ProbeQuant™ G-50 micro columns use spin column chromatography to provide a fast and reliable method for purifying labelled probes from unincorporated labelled nucleotides. In addition, a portion of the eluted DNA can be counted in a scintillation counter and compared to an unpurified sample to give an approximation of percent incorporation. The columns are designed for use in a microcentrifuge, come prepacked with Sephadex™ G-50 DNA grade and are pre-equilibrated in STE, which means that they are ready to use. They can accommodate samples ranging in volume from 25 μl to 50 μl.