623 Results for: "Cy5-SSL-NHS ester&amp"

Supplier: Apollo Scientific

Fmoc-PEG6-NHS ester 50mg pack 1 * 50 mg

Supplier: Apollo Scientific

Ald-PEG4-NHS ester 250mg pack 1 * 250 mg

Supplier: Apollo Scientific

Mal-PEG3-NHS ester 250mg pack 1 * 250 mg

Supplier: Apollo Scientific

Bis-PEG1-NHS ester 250mg pack 1 * 250 mg

Supplier: Apollo Scientific

Bis-PEG4-NHS ester 250mg pack 1 * 250 mg

Supplier: Apollo Scientific

Bis-PEG5-NHS ester 250mg pack 1 * 250 mg

Supplier: Apollo Scientific

Chloroacetamido-PEG4- NHS ester 250mg pack 1 * 250 mg

Supplier: Apollo Scientific

Fmoc-PEG8-NHS ester 250mg pack 1 * 250 mg

Supplier: Apollo Scientific

Biotin-PEG2-NHS ester 100mg pack 1 * 100 mg

Supplier: Apollo Scientific

Bis-PEG7-NHS ester 50mg pack 1 * 50 mg

Supplier: Apollo Scientific

Biotin-PEG3-NHS ester 100mg pack 1 * 100 mg

Supplier: Merck

EMCS and its water-soluble analog Sulfo-EMCS are heterobifunctional cross-linkers that contain N-hydroxysuccinimide (NHS) ester and maleimide groups that allow covalent conjugation of amine- and sulfhydryl-containing molecules. NHS esters react with primary amines at pH 7-9 to form amide bonds, while maleimides react with sulfhydryl groups at pH 6.5-7.5 to form stable thioether bonds. In aqueous solutions, hydrolytic degradation of the NHS ester is a competing reaction whose rate increases with pH. The maleimide group is more stable than the NHS-ester group but will slowly hydrolyze and also lose its reaction specificity for sulfhydryls at pH values > 7.5. For these reasons, conjugation experiments involving these cross-linkers are usually performed at pH 7.2-7.5, with the NHS-ester (amine-targeted) reaction being accomplished before or simultaneous with the maleimide (sulfhydryl-targeted) reaction.

Supplier: Apollo Scientific

Fmoc-PEG2-NHS ester 250mg pack 1 * 250 mg

Supplier: Apollo Scientific

DNP-PEG4-NHS ester 50mg pack 1 * 50 mg

Supplier: Apollo Scientific

Mal-PEG4-NHS ester 50mg pack 1 * 50 mg

Supplier: Apollo Scientific

Biotin-PEG8-NHS ester 50mg pack 1 * 50 mg

Supplier: Apollo Scientific

Fmoc-PEG6-NHS ester 250mg pack 1 * 250 mg

Supplier: Thermo Fisher Scientific

Thermo Scientific EZ-Link Sulfo-NHS-SS-Biotin is a water-soluble, NHS-ester biotinylation reagent whose spacer arm includes a cleavable disulfide bond for reversible labeling of proteins and cell surface primary amines.

Supplier: TriLink BioTechnologies

5-Propargylamino-2'-deoxyuridine can be enzymatically incorporated into DNA and cDNA to introduce primary amine groups. Primary amines can be labeled with NHS ester fluors and haptens for detection and immobilisation of the resulting DNA strand.

Supplier: Thermo Fisher Scientific

Thermo Scientific Pierce SBAP is a short (6.2 angstrom) crosslinker for amine-to-sulfhydryl conjugation via N-hydroxysuccinimide (NHS) ester and bromoacetyl reactive groups.

Supplier: Thermo Fisher Scientific

Thermo Scientific Pierce Sulfo-SANPAH is a hetero-bifunctional crosslinker that contains an amine-reactive N-hydroxysuccinimide (NHS) ester and a photoactivatable nitrophenyl azide.

Supplier: Thermo Fisher Scientific

Thermo Scientific Pierce Sulfo-SANPAH is a hetero-bifunctional crosslinker that contains an amine-reactive N-hydroxysuccinimide (NHS) ester and a photoactivatable nitrophenyl azide.

Supplier: Thermo Fisher Scientific

The amine group is the most common target for modifying protein molecules because it is abundant in the majority of proteins either due to the presence of lysine bearing amino side chain functionality or the N-terminal-amine. Amine-reactive biotinylation reagents can be divided into two groups based on water solubility: NHS-esters and sulfo-NHS-esters.

Supplier: Thermo Fisher Scientific

The amine group is the most common target for modifying protein molecules because it is abundant in the majority of proteins either due to the presence of lysine bearing amino side chain functionality or the N-terminal-amine. Amine-reactive biotinylation reagents can be divided into two groups based on water solubility: NHS-esters and sulfo-NHS-esters.

Supplier: Apollo Scientific

3-(2-Bromoacetamido)propanoic acid nhs ester 250mg pack 1 * 250 mg

Supplier: Merck

The amine-reactive portion of SPDP reagents is the N-hydroxysuccinimide (NHS) ester. Reactions are most commonly performed in phosphate, carbonate/bicarbonate, or borate buffers at pH 7-8. Other buffers can be used provided they do not contain primary amines (or thiols or disulfide reducing reagents – see discussion of the sulfhydryl reactivity). The rate of reaction and degradation by hydrolysis increases with increasing pH; for example, the half-life of the NHS ester is several hours at pH 7 and less than 10 minutes at pH 9. NHS-ester reagents like SPDP and LC-SPDP have limited aqueous solubility and must be dissolved in organic solvent before adding them to a reaction mixture. Sulfo-NHS-ester reagents like Sulfo-LC-SPDP are water-soluble and can be added directly to aqueous reaction mixtures.

Supplier: Thermo Fisher Scientific

Thermo Scientific Pierce BMPS is an amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a short spacer arm (5.9 Å).

Supplier: Thermo Fisher Scientific

Thermo Scientific Pierce SIAB is a mid-length (10.6 angstrom) crosslinker for amine-to-sulfhydryl conjugation via N-hydroxysuccinimide (NHS) ester and iodoacetyl reactive groups.

Supplier: AAT BIOQUEST

BG-NH2 is a carboxy-reactive building block for preparing of SNAP substrates from the NHS esters or other amine-reactive forms of labels or biological targets.

Supplier: Thermo Fisher Scientific

Thermo Scientific Pierce GMBS is an amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a short spacer arm (7.3 angstroms).