Cis-reporting systems, PathDetect
The PathDetect cis-reporting systems is designed for rapid and convenient assessment of the in vivo activation of these pathways. Each cis-reporter plasmid contains the luciferase reporter gene driven by a basic promoter element (TATA box) joined to tandem repeats of various binding elements.
- Identify pathway-specific transcription activation
- Easier and faster than blotting or gel-shift assays
- Sensitive, quantitative assays
When PathDetect cis-reporting and expression plasmids encoding the gene of interest are co-transfected into mammalian cells, increased reporter activity indicates transcription activation and involvement of your gene product in the pathway leading to the enhancer element.
Caution: For research use only. Not for use in diagnostic procedures.
- Pk:1
- Description:PathDetect pTARE-Luc plasmid
- Details:The pTARE-Luc plasmid is incubated at 37 °C for 60 minutes and run on a 0.8% (w/v) agarose gel to test for the absence of contaminating nucleases.
- Contents:—
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