TriLink’s CleanCap® AG (3′ OMe) CleanScript™ IVT kit provides essential components for mRNA synthesis using co-transcriptional capping by in vitro transcription (IVT). The kit components are sufficient to run 25 of 100 µl reactions following our recommended IVT protocol with co-transcriptional capping. It includes a cap analog, modified and unmodified nucleotides, IVT enzymes including CleanScribe RNA polymerase, 10X CleanScript IVT reaction buffer, and a linearized FLuc DNA control template.

The kit is designed for:
Time and cost savings -  Co-transcriptional capping protocol with all necessary reagents for a single-tube reaction providing high yield, fewer steps, and less handling
High capping efficiency - Protocol with CleanCap AG (3′ OMe) cap analog to produce an optimal cap 1 with over 95% capping efficiency
Increased mRNA yield - Buffer and kit components formulated using TriLink’s proprietary CleanScript IVT method for up to 10 mg/ml yield (or up to 1 mg mRNA from 5 µg linear plasmid) per reaction

Ultra-low double-stranded RNA (dsRNA) - up to 85% dsRNA reduction using CleanScribe RNA Polymerase in combination with the CleanScript method
Enhanced mRNA performance - Higher protein expression and lower inflammatory responses from mRNA synthesized with CleanCap AG (3′ OMe), CleanScript method, CleanScribe RNA polymerase, and optional N1-methylpseudouridine.

The kit includes:
CleanCap® Reagent AG (3' OMe), 10 µmol
Adenosine-5'-Triphosphate, 100 µmol
Cytidine-5'-Triphosphate, 100 µmol
Guanosine-5'-Triphosphate, 100 µmol
Uridine-5'-Triphosphate, 100 µmol
N1-Methyl-Pseudouridine-5'-Triphosphate, 100 µmol
AG CleanScribe™ RNA Polymerase Mix, 250 µl
10X AG CleanScript™ IVT Buffer, 250 µl
FLuc Control Plasmid, 25 µg