2'-Fluoro NTPs are being utilised in an increasing number of applications in research and new drug development. 2'-Fluoro-dCTP is incorporated in both DNA and RNA constructs to improve in vivo stability.

• Grade: RUO

Both 2' Fluoro dC and dU are used in the design and synthesis of aptamers, antagomirs and siRNA, because they impart increased target affinity and resistance to endonuclease degradation, while reducing immune response. A key technique utilised in aptamer development using 2'-Fluoro-dCTP is SELEX (Systematic Evolution of Ligands by Exponential Enrichment). The SELEX process allows for the simultaneous screening of 1×1015 different oligonucleotides against a target of interest, such as a protein. The main goal of SELEX is to identify a small subset of aptamers from the original library that bind to the target of interest. Learn more in the technical article: An Overview of the SELEX Process.