The EGFP mRNA expresses an enhanced version of the green fluorescent protein, originally isolated from the jellyfish, Aequorea victoria. EGFP is a commonly used direct detection reporter in mammalian cell culture, yielding bright green fluorescence with an emission peak at 509 nm.
Capped with CleanCap® M6 cap analog, modified with N1-methylpseudouridines to enhance protein expression
Cap 1 structure with >95% capping efficiency for superior in vivo activity compared to Cap-0 mRNAs
Manufactured with CleanCap M6–specific IVT that lowers dsRNA and improves performance
Ready to use in downstream mRNA applications and formulations
Grade: RUO
This mRNA is capped using CleanCap® Reagent M6, TriLink’s co-transcriptional capping technology, resulting in the naturally occurring Cap-1 structure with >95% capping efficiency. It is polyadenylated, modified with N1-methylpseudouridine, and optimised for mammalian systems. It mimics a fully processed mature mRNA.
CleanCap Reagent M6, otherwise known as CleanCap m6AG 3′ OMe, produces a base-modified Cap-1 mRNA, which shows superior in vivo activity compared to Cap-0 mRNA produced by legacy capping methods such as mCap or anti-reverse cap analog (ARCA). CleanCap M6 may further increase protein expression relative to previous generations of cap analogs, such as CleanCap AG or CleanCap AG (3′ OMe), or mRNAs produced by enzymatic capping strategies.
N1-methylpseudouridine is a modified uridine that can reduce immunogenic response and enhance translational efficiency of mRNAs. These properties can result in safer mRNA and increased protein expression.