About this item
The EGFP mRNA expresses an enhanced version of the green fluorescent protein, originally isolated from the jellyfish, Aequorea victoria. EGFP is a commonly used direct detection reporter in mammalian cell culture, yielding bright green fluorescence with an emission peak at 509 nm.
Capped with CleanCap® AG cap analog, no base modifications in the sequence
Cap 1 structure with >95% capping efficiency for superior in vivo activity compared to Cap-0 mRNAs
Manufactured with TriLink’s proprietary CleanScript® method to lower dsRNA for improved performance
Ready to use in downstream mRNA applications and formulations
Grade: RUO
This mRNA is capped using CleanCap® Reagent AG, TriLink's co-transcriptional capping technology, which results in the naturally occurring Cap-1 structure with >95% capping efficiency. It is manufactured using TriLink’s CleanScript® method, polyadenylated, and optimised for mammalian systems. It mimics a fully processed mature mRNA.
CleanCap Reagent AG produces a base-modified Cap-1 mRNA, which shows superior in vivo activity compared to Cap-0 mRNA produced by legacy capping methods such as mCap or anti-reverse cap analog (ARCA).
CleanScript method is TriLink’s proprietary in vitro transcription process that is optimised to minimise dsRNA formation and increase mRNA activity in vivo.