HiTrap™ Chelating HP is prepacked with Chelating Sepharose™ High Performance. The medium is charged with the metalion of your choice for Immobilized Metal ion Affinity Chromatography (IMAC) and subsequent purification of polyhistidine tagged proteins.

• For optimising purification of histidine-tagged proteins using different kinds of metal ions
• Conveniently charge with your metal of choice
• Iminidoacetic acid coupled to Sepharose™ High Performance
• Purifying proteins with exposed histidine groups via metal ion complex formation
• Simple manual operation with a syringe, pump or chromatography system such as ÄKTA design or FPLC system

HiTrap™ Chelating HP is a prepacked ready to use column for preparative affinity chromatography. The special design of the column, together with the matrix, provide fast, simple and easy separations.