Label your antibody with one of our superior CF® Dyes in 30 minutes without a purification step. Labelling tolerates many common buffer components including BSA and ascites.
- Label 5 to 20 µg, 20 to 50 µg, or 50 to 100 µg Ab
- Choice of 29 CF® Dye colours
- Less than 30 seconds of hands-on time
- Total reaction time of 30 minutes
- No purification, 100% recovery
- Compatible with BSA, gelatin, ascites
Mix-n-Stain™ CF® Dye antibody labelling kits allow you to label ≤5 µg up to 100 µg of your antibody in just 30 minutes, with minimal hands-on time and no purification.
Mix-n-Stain™ labeling tolerates common antibody buffer formulations. An ultrafiltration centrifuge vial is provided to quickly remove interfering substances like glycerol, or to concentrate your antibody. With a slight modification in protocol, antibodies can be labelled in the presence of BSA, gelatin, or ascites fluid. Because CF® Dyes are highly water soluble, the presence of other proteins like BSA or gelatin in the labelling reaction has minimal effect on background fluorescence, because any labelled non-antibody proteins readily wash away during immunofluorescence staining.
These kits feature a choice of 32 of Biotium’s next-generation CF® Dyes, which were designed for advantages in brightness and photostability compared to Alexa Fluor®, DyLight®, and other fluorescent dyes.
Mix-n-Stain™ antibody labelling kits are very simple to use (see the workflow overview). But before you begin, you must check that your antibody meets the compatibility requirements for labelling, and choose the right labelling protocol. See the product table below for a quick reference of the maximum excitation and emission of each CF® Dye.
Biotium now offer Mix-n-Stain™ Maxi Antibody labelling kits for labelling 1 mg of antibody with CF® Dyes, and Mix-n-Stain™ Nanobody labelling kits. Mix-n-Stain™ kits also are available for labelling antibodies with enzymes (HRP, AP, and GOx), fluorescent proteins (PE, APC, PerCP, and tandem dyes), biotin, or DIG.