Specifications
- Description:EasySep™ release human CD19 positive selection kit, 1×10⁹ cells
- Pk:1 KIT
Specifications
About this item
Immunomagnetic positive selection of particle-free human CD19+ cells.
- Highly purified human CD19+ cells isolated in less than 30 minutes
- No-wash removal of EasySep™ Releasable RapidSpheres™
The EasySep™ Release Human CD19 Positive Selection Kit is designed to isolate CD19+ cells from fresh or previously frozen peripheral blood mononuclear cells or washed leukapheresis samples by immunomagnetic positive selection.
Desired cells are labelled with antibodies and magnetic particles, and separated without columns using an EasySep™ magnet. Unwanted cells are simply poured off, while desired cells remain in the tube. Then, bound magnetic particles are removed from the EasySep™-isolated CD19+ cells, which are immediately available for downstream applications. Following cell isolation with this EasySep™ Release kit, antibody complexes remain bound to the cell surface and may interact with Brilliant Violet™ antibody conjugates, polyethylene glycol-modified proteins or other chemically related ligands. The CD19 antigen is expressed on mature B cells and B cell progenitors but is lost upon maturation to plasma cells. It is also expressed on follicular dendritic cells.