Defined supplement for improving survival of human ES and iPS cells in single-cell workflows.

Generate clonal human pluripotent stem cell (hPSC) lines that maintain their genomic integrity and downstream differentiation potential with this supplement. Using CloneR™2 will increase the cloning efficiency and survival of human embryonic stem (ES) and induced pluripotent stem (iPS) cells under high-stress conditions, including seeding at low or high densities. For gene-editing workflows, add CloneR™2 to improve ES and iPS cell survival following electroporation and during clonal deposition. It also facilitates the survival and expansion of clonal lines generated by low-density seeding (<25 cells/cm²) or by FACS-sorting cells at 1 cell/well, without requiring single-cell adaptation. Also use CloneR™2 supplement to increase survival of single cells plated at higher densities, including under stressful conditions such as the post-thaw recovery of ES and iPS cell lines and when creating monolayers ahead of downstream differentiation. Defined and serum-free, CloneR™2 supplement is compatible for use with TeSR™ ES and iPS cell maintenance media, as well as a choice of cell culture matrix.