Serum-free and conditioned medium-free cell culture medium for establishment and maintenance of human intestinal organoids.

IntestiCult™-SF Organoid Growth Medium (Human) is a complete cell culture medium for efficient establishment and long-term maintenance of organoids derived from human intestinal crypts. IntestiCult™-SF is a serum-free and conditioned medium-free formulation. Intestinal organoids provide a convenient in vitro organotypic culture system for studying the intestinal epithelium. Isolated intestinal crypts rapidly form complex organoids when cultured in IntestiCult™-SF. The organoids incorporate a functional lumen enclosed by a polarised epithelial cell layer containing known cell types of the adult intestinal epithelium. Maturing intestinal organoids establish protruding buds, resembling intestinal crypts of the in vivo tissues.

IntestiCult™-SF supports the proliferation of intestinal epithelial stem cells from patient’s biopsies and expansion of organoid cultures derived from fresh tissue or from already established frozen stocks. Applications of intestinal organoid cultures include studying the development and function of intestinal epithelium, modeling intestinal diseases, and performing targeted molecule screens. Intestinal organoid cultures can also be used for investigation of adult stem cell properties and for regenerative therapy approaches, and can be further differentiated using IntestiCult™ Organoid Differentiation Medium.