For differentiation of human ES or iPS cells into erythroid progenitor cells.

STEMdiff™ Erythroid Kit is designed for the serum-free and feeder-free differentiation of human embryonic stem (ES) and induced pluripotent stem (iPS) cells to erythroid progenitor cells (erythroblasts) expressing Glycophorin A and CD71. This simple 2D differentiation protocol is performed in two stages. During the first 10 to 12 day stage, STEMdiff™ Hematopoietic Supplement A and then Supplement E1 are added to basal medium to induce cells toward erythroid-biased hematopoietic progenitor cells. At the end of this stage, hematopoietic progenitor cells are easily harvested from the culture supernatant. During the second 14-day stage, cells are further differentiated to erythroid progenitor cells using Supplement E2 and StemSpan™ SFEM II (Day 10 to 24).

The cells typically expand 241-fold +/- 190-fold (95% CI) during the 14-day erythroid differentiation stage and the Day 24 cell population typically contains 74% +/- 5% (95 CI) CD71+GlyA+ erythroblasts. Cells generated using STEMdiff™ Erythroid Kit can be further matured into normoblasts and reticulocytes when moved to appropriate culture conditions for maturation. STEMdiff™ Erythroid Kit has been optimised for differentiation of cells maintained in mTeSR™1, mTeSR™ Plus, and TeSR™-E8™.