Serum-free medium kit for highly efficient SMAD inhibition-mediated neural induction of human ES and iPS cells.

STEMdiff™ SMADi neural induction kit consists of a defined, serum-free medium and supplement for the highly efficient neural induction of human embryonic stem (ES) cells and induced pluripotent stem (iPS) cells. This kit combines STEMdiff™ neural induction medium with STEMdiff™ SMADi neural induction supplement, which directs differentiation by blocking TGF-β/BMP-dependent SMAD signaling, resulting in efficient neural induction of even hard-to-differentiate cell lines. Neural progenitor cells (NPCs) can be generated using STEMdiff™ SMADi neural induction kit with either an embryoid body (EB) protocol or monolayer culture protocol. The resulting cultures are enriched for central nervous system (CNS)-type NPCs, which express SOX1, Nestin, and PAX6. NPCs generated using this kit can be passaged as single cells and expanded in STEMdiff™ neural progenitor medium. The NPCs can also be differentiated into neurons and glia.Learn how to generate neural progenitor cells from human pluripotent stem cells (hPSCs) in our on-demand neural induction course, and browse our tech tips on the neural induction of hPSCs using the embryoid body method or monolayer method.