Specifications
- Pk:1 KIT
- Assay Type:Competitive
- Format:Pre-coated
- Primary antibody reactivity:Species independent
- Target protein:cAMP
- Description:cAMP ELISA kit
- Cross reactivity:cyclic AMP (100%)AMP, ATP, cyclic GMP, GMP, GTP, cyclic UMP, CTP (<0,001%)
- Detection method:Colorimetric 450 nm
- Storage temperature:4 °C (long term), −20 °C (standard and conjugate)
- Sensitivity:Non-acetylated: 0,49 pmol/ml (range 0,78 - 200 pmol/ml) Acetylated: 0,027 pmol/ml (range 0,078 - 20 pmol/ml)
- Regulatory status:RUO
Specifications
About this item
Most sensitive (0,027 pmol/ml) colorimetric kit for extracellular cAMP quantification.
- Sensitive – optional acetylation protocol increases sensitivity >10-fold (0,027 pmol/ml)
- Rapid – results in just three hours from up to 39 samples in duplicate
- Easy-to-use – pre-coated plate and liquid color-coded reagents reduce errors
- Simple – efficient and well-established sample handling protocols
- Reliable – consistent lot-to-lot performance
This is a colorimetric competitive enzyme immunoassay kit for the quantitative determination of extracellular cAMP. Sensitivity is increased >10-fold by acetylation (reagents included). Absorbance is read at 405 nm. The ready-to-use liquid color-coded reagents provided reduce error.
Cyclic AMP (cAMP) is one of the most important "second messengers" involved as a modulator of physiological processes. A number of hormones are known to activate cAMP through the action of the enzyme Adenylate cyclase which converts ATP to cAMP. cAMP has been shown to be involved in the cardiovascular and nervous systems, immune mechanisms, cell growth and differentiation, and general metabolism.