DEAE Sephadex™ A-50 Ion Exchange Chromatography Media, Cytiva

DEAE Sephadex™ A-25 and DEAE Sephadex™ A-50 are weak anion exchangers. Sephadex™ ion exchangers are produced by introducing functional groups onto the cross-linked dextran matrix. These groups are attached to glucose units in the matrix by stable ether linkages.

Weak anion exchanger suitable for batch techniques
High binding capacities
Well-proven and widely used
Stable to all commonly used aqueous buffers additives such as 8 M urea and 6 M guanidine hydrochloride

These groups are attached to glucose units in the matrix by stable ether linkages. Different types of ion exchangers (DEAE, QAE and CM) and two different porosities (A-25/C-25 and A-50/C-50) are available.

Sephadex ion exchangers are produced by introducing functional groups onto the cross-linked dextran matrix.

pH range:
2 - 9
Matrix:
Cross-linked dextran
Ligand:
Diethylaminoethyl
Storage temperature:
4 - 30 °C
Binding capacity:
Thyroglobulin (Mr 669 000)- 2 mg/ml drained resin), HSA (Mr 68 000)- 110 mg/ml drained resin), α-lactalbumin (Mr 14 300)- 50 mg/ml drained resin)
Total ionic capacity:
3-4 mmol/g
Description:
DEAE Sephadex™ A-50