A sandwich ELISA kit for the quantitative measurement of osteocalcin in mouse serum, plasma and other biological fluids.

• Sensitivity: 0,23 ng/ml
• Specificity: no significant cross-reactivity or interference between mouse OCN and analogues was observed
• Variation: coefficient of variation is <10%
• Detection range: 0,39 to 25 ng/ml

Osteocalcin (OCN) is one of the key players in bone endocrinology. It is expressed and secreted solely by osteoblasts. Following protein synthesis, the mature peptide first undergoes several splicing events and then gets γ-carboxylated at three residues, resulting in a peptide with high affinity toward bone and the extracellular matrix. OCN also acts as a hormone impacting glucose metabolism and insulin secretion. Osteocalcin (mouse) ELISA kit is based on sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to mouse osteocalcin alpha. Standards or samples are added to the micro ELISA plate wells that bind to the specific antibody. Then a biotinylated detection antibody specific for mouse osteocalcin and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. The wells are washed, a TMB substrate solution is added to the wells and blue color develops in proportion to the amount of Osteocalcin bound. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ±2 nm. The OD value is proportional to the concentration of mouse osteocalcin. The concentration of mouse osteocalcin in the samples can be calculated by comparing the OD of the samples to the standard curve.