A sandwich ELISA kit for the quantitative measurement of TNF-alpha in biological fluids.

• Easy, convenient and time-saving method
• Detection Range: 15,6 to 1000 pg/ml
• Sensitivity: <1 pg/ml
• Assay precision: Intra-assay: CV <8%, inter-assay: CV <10%
• No significant cross-reactivity or interference was observed

Tumor necrosis factor-α (TNF-α) is a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells and certain other target cells. TNF is a cytokine involved in systemic inflammation and is a member of a group of cytokines that all stimulate the acute phase reaction.

TNF is mainly secreted by macrophages. TNF causes apoptotic cell death, cellular proliferation, differentiation, inflammation, tumorigenesis and viral replication, TNF is also involved in lipid metabolism, and coagulation.

TNF's primary role is in the regulation of immune cells. Dysregulation and, in particular, overproduction of TNF have been implicated in a variety of human diseases- autoimmune diseases, insulin resistance, and cancer.

TNF-alpha ELISA kit is a sandwich ELISA assay for the quantitative measurement of TNF-alpha in human serum, plasma and cell culture supernatants. The density of colour is proportional to the amount of TNF-alpha captured from the samples.

All products are FOR RESEARCH USE ONLY.   PRODUCTS ARE NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES.  Customer is granted a limited use non-exclusive licence for its own internal research.  Any control manufacturer supplies with a Product is limited to use only to validate the relevant Product.  By using the Product, Customers agree not to attempt to reverse engineer or otherwise perform any compositional, structural, functional or other analyses directed to learning the methodology, formulae, sequences, processes, make-up or production of any of the Products or portions thereof.