The MycoAlert™ PLUS mycoplasma detection kit is the next generation Lonza MycoAlert™ kit. It provides higher light output to allow for use with less sensitive plate luminometers or multifunctional readers and testing of unused media, media supplements or water.

• Convenient and fast
• Simply add two reagents to the culture supernatant, perform two luminescence readings and get your results in less than 20 minutes
• Detects all common mollicute contaminations (mycoplasma, acholeplasma, entomoplasma and spiroplasma; except ureaplasma)
• Suited for tube luminometers, plate luminometers and multifunctional readers

The MycoAlert™ PLUS assay is a selective biochemical test that exploits the activity of mycoplasmal enzymes which are found in all six of the main mycoplasma cell culture contaminants and the vast majority of 180 mycoplasma species, but are not present in eukaryotic cells.

MycoAlert™ Assay Control Set (available separately)
The MycoAlert™ assay control set includes a lyophilised positive control and assay buffer for reconstitution. The assay buffer also serves as a negative control. The positive and negative controls are designed for use with the MycoAlert™ PLUS mycoplasma detection kit. It is recommended to always include the control set to ensure that the operator and all of the assay reagents are performing properly. The positive control is proprietary, but does NOT contain mycoplasma. The positive or negative control is simply substituted for a sample in the standard assay protocol. The positive control will give a ratio of >1 while the negative control will give a ratio of <1.

Viable mycoplasma in a test sample (cell supernatant or fresh media or supplements) are lysed and the enzymes react with the MycoAlert™ PLUS substrate, catalysing the conversion of ADP to ATP. The ATP is then transferred into a light signal via the luciferase enzyme in the MycoAlert™ PLUS Reagent. By measuring the level of ATP in a sample both before (read A) and after the addition of the MycoAlert™ PLUS substrate (read B), a ratio can be obtained which is indicative of the presence or absence of mycoplasma.