The PathDetect cis-reporting systems is designed for rapid and convenient assessment of the in vivo activation of these pathways. Each cis-reporter plasmid contains the luciferase reporter gene driven by a basic promoter element (TATA box) joined to tandem repeats of various binding elements.

• Identify pathway-specific transcription activation
• Easier and faster than blotting or gel-shift assays
• Sensitive, quantitative assays

When PathDetect cis-reporting and expression plasmids encoding the gene of interest are co-transfected into mammalian cells, increased reporter activity indicates transcription activation and involvement of your gene product in the pathway leading to the enhancer element.