Specifications

• Pk:
  100 µG
• Conjugation:
  Unconjugated
• Protein/peptide type:
  Recombinant
• Source:
  HEK293 cells
• Species:
  Human
• Tag sequence:
  His Tag
• Storage conditions:
  –20 °C
• Endotoxin content:
  Less than 0,01 EU per μg by the LAL method.
• Biological activity:
  Measured by its binding ability in a functional ELISA. Immobilized Human PD-1, His Tag, low endotoxin (HPLC-verified) (Cat. No. PD1-H522a) at 2 μg/mL (100 μL/well) can bind Human PD-L2, Mouse IgG1 Fc Tag (Cat. No. PD2-H52A5) with a linear range of 10-156 ng/mL (Routinely tested). Loaded Human PD-1, His Tag, low endotoxin (Cat. No. PD1-H522a) on HIS1K Biosensor, can bind Human PD-L1, Fc Tag (Cat. No. PD1-H5258) with an affinity constant of 23.3 nM as determined in BLI assay (ForteBio Octet Red96e) (QC tested). Loaded Human PD-1, His Tag, low endotoxin (Cat. No. PD1-H522a) on HIS1K Biosensor, can bind Human PD-L2, Fc Tag (HPLC-verified) (Cat. No. PD2-H5251) with an affinity constant of 15.7 nM as determined in BLI assay (ForteBio Octet Red96e) (QC tested). Loaded Human PD-L1, Fc Tag (Cat. No. PD1-H5258) on Protein A Biosensor, can bind Human Human PD-1, His Tag, low endotoxin (Cat. No. PD1-H522a) with an affinity constant of 4.8 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested). Loaded Human PD-L2, Fc Tag (HPLC verified) (Cat. No. PD2-H5251) on Protein A Biosensor, can bind Human Human PD-1, His Tag, low endotoxin (Cat. No. PD1-H522a) with an affinity constant of 0.9 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
• Gene ID:
  Q15116
• Protein synonyms:
  CD279|PDCD1|PD1|SLEB2
• Protein/peptide name:
  PD-1
• Purity:
  >95% as determined by SDS-PAGE.
• Molecular weight:
  16,8 kDa
• Endotoxin level:
  Low
• Formulation:
  Lyophilised from 0,22 μm filtered solution in PBS, pH 7,4. Normally trehalose is added as protectant before lyophilisation.
• Shipping temperature:
  RT