Specifications

• Conjugation:
  Unconjugated
• Protein/peptide type:
  Recombinant
• Source:
  HEK293 cells
• Species:
  Human
• Tag sequence:
  His Tag (SPR verified)
• Storage conditions:
  –20 °C
• Endotoxin content:
  Less than 1,0 EU per μg by the LAL method.
• Biological activity:
  Measured by its binding ability in a functional ELISA. Immobilised Human Insulin R (28-944), His Tag at 5 μg/ml (100 μl/well) can bind Human IGF-II, Fc Tag with a linear range of 0,078 to 1,25 μg/mL (QC tested). Measured by its binding ability in a functional ELISA. Immobilised Human Insulin R (28-944), His Tag at 5 μg/ml (100 μl/well) can bind Insulin Biotin Conjugate with a linear range of 0,02 to 0,313 μg/ml (Routinely tested). Human Insulin R (28-944), His Tag captured on CM5 chip via anti-His antibody, can bind Human Insulin with an affinity constant of 249 nm as determined in a SPR assay (Biacore T200) (QC tested).
• Gene ID:
  P06213-2
• Protein synonyms:
  INSR|Insulin receptor|IR|CD220
• Protein/peptide name:
  Insulin R
• Purity:
  >90% as determined by SDS-PAGE.
• Molecular weight:
  106,5 kDa
• Endotoxin level:
  Low
• Formulation:
  Lyophilised from 0,22 μm filtered solution in PBS, pH7,4. Normally trehalose is added as protectant before lyophilisation.
• Shipping temperature:
  RT