Unconjugated

Recombinant

HEK293 cells

Human

–20 °C

Less than 1,0 EU per μg by the LAL method.

Lyophilised from 0,22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH 7,5. Normally trehalose is added as protectant before lyophilisation. Measured by its binding ability in a functional ELISA. Immobilised human LAG-3, mouse IgG2a Fc Tag (Cat. No. ACRBLA3-H52Aa) at 2 μg/ml (100 μl/well) can bind anti-human LAG3 MAb, human IgG1 with a linear range of 1 - 10 ng/ml (QC tested). Measured by its binding ability in a functional ELISA. Immobilised human LAG-3, mouse IgG2a Fc Tag (Cat. No. ACRBLA3-H52Aa) at 10 μg/ml (100 μl/well) can bind cynomolgus / rhesus macaque FGL1, Fc Tag (Cat. No. ACRBFG1-C5269) with a linear range of 0,01 - 0,156 μg/ml (routinely tested). Flow cytometry assay shows that Human LAG3, Mouse IgG2a Fc Tag (Cat. No. ACRBLA3-H52Aa) can bind to daudi cell surface. The concentration of LAG3 used is 0,3 μg/ml (routinely tested). FACS analysis shows that the binding Human LAG3, mouse IgG2a Fc Tag (Cat. No. ACRBLA3-H52Aa) to daudi cells was inhibited by increasing concentration of neutralising Anti-LAG3 antibody. The concentration of LAG3 used is 1 μg/ml. The IC50 is 0,165 μg/ml (routinely tested).

P18627-1

Y

Y

Y

FDC|CD223|LAG3

LAG-3

>95%

73,3 kDa

Low

Lyophilised from 0,22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH 7,5. Normally trehalose is added as protectant before lyophilisation.

Y

Powder,RT