Human recombinant Fc gamma RIIB/ CD32b (from HEK293 cells)
- Conjugation:Unconjugated
- Protein/peptide type:Recombinant
- Source:HEK293 cells
- Species:Human
- Tag sequence:His Tag (SPR & BLI verified)
- Storage conditions:–20 °C
- Endotoxin content:Less than 1,0 EU per μg by the LAL method.
- Biological activity:Immobilised Human CD32b, His Tag (SPR & BLI verified) (Cat. No. CDB-H5228) on CM5 Chip via anti-His antibody, can bind Rituximab with an affinity constant of 10,1 μM as determined in a SPR assay (Biacore T200) (QC tested). ; Immobilised Rituximab on CM5 Chip, can bind Human CD32b, His Tag (SPR & BLI verified) (Cat. No. CDB-H5228) with an affinity constant of 5,5 μM as determined in a SPR assay (Biacore T200) (Routinely tested). ; Loaded Human CD32b, His Tag (SPR & BLI verified) (Cat. No. CDB-H5228) on HIS1K Biosensor, can bind Rituximab with an affinity constant of 4,30 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested). Loaded Rituximab on FAB2G Biosensor, can bind Human CD32b, His Tag (SPR & BLI verified) (Cat. No. CDB-H5228) with an affinity constant of 5,4 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
- Gene ID:P31994
- Protein synonyms:CD32|IGFR2|CD32b|FCG2|CDw32|FCGR2B
- Protein/peptide name:Fc gamma RIIB/ CD32b
- Purity:>95% as determined by SDS-PAGE.
- Molecular weight:20,4 kDa
- Endotoxin level:Low
- Formulation:Lyophilised from 0,22 μm filtered solution in PBS, pH 7,4. Normally trehalose is added as protectant before lyophilisation.
- Shipping temperature:RT