Human recombinant M-CSF (from HEK293)
- Conjugation:Unconjugated
- Protein/peptide type:Recombinant
- Source:HEK293 cells
- Species:Human
- Storage conditions:–20 °C
- Endotoxin content:Less than 1,0 EU per μg by the LAL method.
- Biological activity:Lyophilised from 0,22 μm filtered solution in PBS, pH 7,4. Normally trehalose is added as protectant before Lyophilisation.; Measured by its binding ability in a functional ELISA. Immobilised Human M-CSF, His Tag (Cat. No. MCF-H5247) at 5 μg/ml(100 μl/well) can bind Human M-CSF R, Fc Tag (Cat. No. CSR-H5258) with a linear range of 0.1-4 ng/ml(QC tested).; Measured by its binding ability in a functional ELISA. Immobilised Human M-CSF, His Tag (Cat. No. MCF-H5247) at 5 μg/ml(100 μl/well) can bind Cynomolgus M-CSF R, Fc Tag (Cat. No. CSR-C5252) with a linear range of 1-39 ng/ml(Routinely tested).; Human M-CSF, His Tag (Cat. No. MCF-H5247) captured on CM5 Chip via anti-His antibody can bind Human M-CSF R, Fc Tag, low endotoxin (Cat. No. CSR-H5258) with an affinity constant of 1.9 nM as determined in SPR assay (Biacore T200) (Routinely tested).; Loaded Human M-CSF, His Tag (Cat. No. MCF-H5247) on HIS1K Biosensor, can bind Human M-CSF R, Fc Tag, low endotoxin (Cat. No. CSR-H5258) with an affinity constant of 3.29 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).; Loaded Human M-CSF R, Fc Tag, low endotoxin (Cat. No. CSR-H5258) on Protein A Biosensor, can bind Human M-CSF, His Tag (Cat. No. MCF-H5247) with an affinity constant of 0.365 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
- Gene ID:P09603-1
- Carrier-free:Y
- Protease-free:Y
- Animal-free:Y
- Protein synonyms:CSF-1|M-CSF|Lanimostim
- Protein/peptide name:M-CSF
- Purity:>95%
- Molecular weight:26,0 kDa
- Endotoxin level:Low
- Formulation:Lyophilised from 0,22 μm filtered solution in PBS, pH 7,4. Normally trehalose is added as protectant before Lyophilisation.
- Nuclease-free:Y
- Shipping temperature:Powder,RT