Enhanced chemiluminescence (ECL) detection reagents for reliable detection of medium and high expression proteins.
- Ideal for confirmatory Western blotting applications using chemiluminescence detection
- At least 10-fold more sensitive than colorimetric assays
- Detectable on Amersham Hyperfilm ECL or with a CCD imager such as ImageQuant LAS 500.
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High sensitivity chemiluminescence detection on Western blotting
Amersham ECL Western Blotting Detection Reagents use enhanced luminol-based detection, which is suitable for all routine Western blotting. ECL Western Blotting Detection Reagents, in combination with Amersham Protran membranes, form a complete solution for Western blot applications.
Reliable chemiluminescence detection
In most contemporary ECL systems a luminol peroxide detection reagent is added to the membrane and reacts with the horseradish peroxidase enzyme (HRP) conjugated to the secondary antibody. HRP catalyzes the oxidation of luminol in a multistep reaction and is accompanied by the emission of low-intensity light at 428 nm, which can be measured with light-sensitive X-ray film or with a CCD imager.