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Trypsin

Trypsin

100 mL
Catalog # PANBP10-036100
Marketsource
Supplier:   PAN BIOTECH
Trypsin
Catalog # PANBP10-036100
Supplier:  PAN BIOTECH
Marketsource
CAS Number:  
Trypsin

100 mL

Specifications
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Specifications
CAS Number:  
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1 * 100 mL
Product Details & Documents

Trypsin is a porcine pancreas-derived enzyme that is commonly usedin dissociation of anchorage dependent mammalian cells and tissues.

  • Stability: two years from production

The concentration of trypsin is necessary to dislodge cells from their substrate is dependent on the cell type and age of the culture. EDTA, a chelating reagent is added to trypsin solution to enhance enzymatic activity by neutralising calcium and magnesium ions that enhance cell to cell adhesion and obscure peptide bonds on which trypsin acts.

Specifications

Specifications

  • Pk:
    100 mL
  • Cat. No.:
    PANBP10-036100
  • Form:
    Solution
  • Storage conditions:
    −20 °C
  • Concentration:
    0,25% trypsin/0,53 mM EDTA in HBBS, without calcium and magnesium, with phenol red
  • Enzyme name:
    Trypsin

Specifications

   
Acetonitrile, anhydrous (max. 0.003% H₂O) ≥99.95%, HiPerSolv CHROMANORM® Reag. USP, Reag. Ph. Eur., ACS, super gradient grade suitable for UPLC/UHPLC instruments

  #: 83639.320

Supplier: VWR Chemicals

Methanol ≥99.8%, HiPerSolv CHROMANORM® Reag. Ph. Eur., gradient grade for HPLC

  #: 20864.320

Supplier: VWR Chemicals

VWR®, Transfer Pipettes, High Performance

  #: 612-1681

Supplier: VWR Collection

Acetone ≥99%, TECHNICAL

  #: 20063.365

Supplier: VWR Chemicals

VWR®, Nitrile Gloves

  #: 112-2371

Supplier: VWR Collection

VWR® Serological Pipette, Standard Line

  #: 612-3700

Supplier: VWR Collection

VWR® NITRILE LIGHT, Nitrile Gloves

  #: 112-2756

Supplier: VWR Collection

VWR® Facial Tissues

  #: 115-0600

Supplier: VWR Collection

Acetonitrile ≥99.9%, HiPerSolv CHROMANORM® for LC-MS, suitable for UPLC/UHPLC instruments

  #: 83640.320

Supplier: VWR Chemicals

Nitrile gloves, Kimtech™ Purple Nitrile™

  #: 112-4847

Supplier: Ansell

Acetonitrile ≥99.9%, LiChrosolv® Reag. Ph. Eur., gradient grade for liquid chromatography, Supelco®

  #: 1.00030.2500

Supplier: Merck

pH indicator strips, MQuant®, Supelco®

  #: 1.09535.0001

Supplier: Merck

Sealing film for manual application, Parafilm® M

  #: 291-0057

Supplier: HEATHROW

Water, HiPerSolv CHROMANORM® for HPLC

  #: 23595.328

Supplier: VWR Chemicals

Sodium hydroxide 0.1 mol/l (0.1 N) in aqueous solution, AVS TITRINORM Reag. Ph. Eur. volumetric solution

  #: 31770.294

Supplier: VWR Chemicals

VWR® AVS TITRINORM Buffer Solutions, 20 °C

  #: 32096.291

Supplier: VWR Chemicals

Water, HiPerSolv CHROMANORM® for LC-MS, suitable for UPLC/UHPLC instruments

  #: 83645.320

Supplier: VWR Chemicals

VWR® Nitrile Extra Light, Nitrile Gloves

  #: 112-4195

Supplier: VWR Collection

Buffer solutions, ready to use, referenced at 20 °C, Certipur®, Supelco®

  #: 1.09439.1000

Supplier: Merck

epT.I.P.S.® Bulk - Pipette Tips

  #: 613-3508

Supplier: EPPENDORF