Prostaglandin Screening ELISA kit is used for the quantification of total PG levels in tissue culture medium.

• Assay 24 samples in triplicate or 36 samples in duplicate
• Measure total PG levels down to 30 pg/ml
• Read: Colorimetric at 405 to 420 nm

This assay was developed for screening applications in which the relative amount of prostaglandin (PG) production for a large number of cell culture samples must be determined. Our PGE₂ assay (Item No.514010) is unparalleled in sensitivity and specificity, but the steep standard curve can make screening many samples tedious.

The specificity of the PGE₂ assay also eliminates quantification of other PGs which may be produced by the cells. The antiserum used in this assay exhibits high cross reactivity for most PGs which will allow quantification of all the PGs in a given sample with a single assay.

The broad standard curve minimizes the need for sample dilution. Because this assay will also recognize thromboxane B₂ (TXB₂), cell culture media must not contain fetal calf serum (FCS). The high concentration of TXB₂ in serum (approximately 400 ng/ml) will completely displace the tracer, making PG measurement impossible.