Cholesterol Cell-Based Detection Assay Kit is a 192-well assay for measurement of cholesterol.

• Detect cholesterol uptake and localisation within cultured cells
• Employs filipin III, a probe that fluoresces in the presence of cholesterol
• An inhibitor of cholesterol trafficking is included as a positive control

The mechanism for the movement of cholesterol from intracellular sites to their ultimate cellular destination is an unresolved question of fundamental importance to cell biology and medicine. Thus, defining mechanisms of intracellular cholesterol transport and identifying the cellular factors involved are therefore of great interest. The Cholesterol Cell-Based Detection Assay Kit provides a simple fluorometric method to study mechanisms and biological factors that regulate cholesterol metabolism or movement within cells.