Prostaglandin D₂ Express ELISA kit is a competitive assay that can be used for quantification of PGD₂.

• Measure PGD₂ in multiple sample types
• Assay 24 samples in triplicate or 36 samples in duplicate
• Measure PGD₂ levels down to 350 pg/ml
• Get results in under 4 hours

Prostaglandin D₂ (PGD₂) is biosynthesised in the brain by a soluble, 26 kDa glutathione-independent lipocalin-type PGD₂ synthase. PGD₂ accumulates in the cerebrospinal fluid (CSF), where it induces physiologic sleep in rats and humans. PGD₂ is also synthesised in mast cells and leukocytes by a cellular, myeloid-type, glutathione-dependent PGD synthase. This PGD₂ which is formed in the intracellular and vascular compartments is rapidly metabolised to 11β-PGF2α and other metabolites.

Measurement of the parent eicosanoid PGD₂ is appropriate in cell culture lysates and in CSF, where concentrations of several hundred pg/ml have been measured. PGD₂ Express ELISA Kit is a competitive assay that can be used for quantification of PGD₂ in cell culture lysates and enzymatic reactions. This ELISA offers the convenience of a fast assay with a two hour incubation time and one hour development time, while still achieving an assay range of approximately 117,2 to 15000 pg/ml and a sensitvity (80% B/B₀) of approximately 350 pg/ml.