Laminin is an extracellular matrix multidomain trimeric glycoprotein, and is the main non-collagenous component of basal lamina that supports adhesion, proliferation and differentiation.

• Filtered at 0,2 μm
• Supplied at a concentration of 1 mg/ml in 50 mM Tris-HCl, with 150 mM NaCl

Laminin is composed of both A, B1 and B2 chains, which are connected by many disulfide bonds. This laminin product was isolated from mouse Engelbreth-Holm-Swarm tumor. It supports growth and differentiation of many cell types including epithelial, endothelial, neural, muscle and liver cells. It is recommended for use as a cell culture substratum at one to two μg/cm².

It has been used in insulin-producing cell (IPC) differentiation, infection inhibition assays and proliferation assay. Thaw this solution slowly before use at 2 to 8 °C to avoid gel formation. For use as a coating, dilute in a balanced salt solution, coat culture surface with a minimal volume and incubate at 37 °C for two hours. Wash 3 times with PBS before plating cells.