Terminal labelling kit with biotin-ddUTP, BIOARRAY™

BIOARRAY™ terminal labelling kit with Biotin-ddUTP has been developed for DNA probe array assays.

This method uses Bio-ddUTP and terminal deoxynucleotide transferase to catalyse the addition of a single biotin-ddUMP to the 3'-OH terminus of an amplified and fragmented target DNA molecule. A standard reaction will label up to 100 picomoles (equivalent to 1 µg of a 30-nucleotide sequence). When DNA fragments are labelled at the 3'-OH terminus, the sequence bias that occurs with either nick translation or random priming is eliminated.

Delivery information: Supplied with Biotin-ddUTP (100X), 25 µl, reaction buffer (5X), potassium cacodylate buffer (500 µl), pH 7, containing beta-mercaptoethanol and stabiliser, 10X CoCl₂ solution (250 µl) and terminal deoxynucleotide transferase (50X), 50 µl in storage buffer.