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Human recombinant Carbonic Anhydrase 4 (from E. coli)
  PRSI91-092
 :  
Human recombinant Carbonic Anhydrase 4 (from E. coli)
  PRSI91-092
 :  

 

  • Pk:
    50 µG
  • Enzyme type:
    Recombinant
  • Source:
    E. coli
  • Species:
    Human
  • Tag sequence:
    C-6 His tag
  • Storage conditions:
    Store at −20 °C, stable for 6 months after receipt. Please minimize freeze−thaw cycles.
  • Enzyme name:
    Carbonic Anhydrase 4
  • Purity:
    >95% as determined by reducing SDS-PAGE
  • Molecular weight:
    31.43 kD
  • Sequence:
    Ala19-Lys283
  • Formulation:
    Supplied as a 0.2 µm filtered solution of 20 mM TrisHCl, 100 mM NaCl, pH 8.5. Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
  • Tested applications:
    Bioassay

 

 

Carbonic Anhydrase 4 (CA4) belongs to the alpha-carbonic anhydrase family. Alpha-carbonic anhydrase is a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. Carbonic anhydrase 4 is a glycosylphosphatidyl-inositol-anchored membrane isozyme expressed on the luminal surfaces of pulmonary (and certain other) capillaries and proximal renal tubules. Carbonic anhydrase 4 may stimulate the sodium/bicarbonate transporter activity of SLC4A4 that acts in pH homeostasis. It may have a role in inherited renal abnormalities of bicarbonate transport. Furthermore, Carbonic anhydrase 4 is essential for acid overload removal from the retina and retina epithelium and acid release in the choriocapillaris.

Fusion-Tag: C-6 His tag

This recombinant protein can be used for biological assays. For research use only.