Recognizes a protein of ~76 kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly.This MAb can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.
Type: Primary
Antigen: NCL
Clonality: Monoclonal
Clone: 364-5
Conjugation: CF405S
Epitope:
Host: Mouse
Isotype: IgG1 kappa
Reactivity: Human
- Catalog No:
- BNC040527-100
- BNC040527-500
- Antigen symbol:
- NCL
- NCL
- Antigen name:
- Nucleolin
- Nucleolin
- Antigen synonyms:
- Protein C23|Nucleolin|NCL|Nucl
- Protein C23|Nucleolin|NCL|Nucl
- Antibody type:
- Primary
- Primary
- Clonality:
- Monoclonal
- Monoclonal
- Conjugation:
- CF® 405S
- CF® 405S
- Clone:
- Clone 364-5
- Clone 364-5
- Reactivity:
- Human
- Human
- Host:
- Mouse
- Mouse
- Gene ID:
- 79110
- 79110
- Isotype:
- IgG1 kappa
- IgG1 kappa
- Western blot:
- Yes
- Yes
- ImmunoChemistry:
- Yes
- Yes
- ImmunoFluorescence:
- Yes
- Yes
- Concentration:
- 0,1 mg/ml
- 0,1 mg/ml
- Storage buffer:
- PBS/0,1% BSA/0,05% azide
- PBS/0,1% BSA/0,05% azide