CHOD is soluble in cold 50 mM potassium phosphate buffer, pH 7,0. Prepare solutions immediately before use.

One unit causes the formation of one µmole of hydrogen peroxide per minute at pH 7,0 and 37 °C.

Stabilisers: Bovine serum albumin, sugars. Typical Contaminants: Catalase: Not more than 1,0% Cholesterol esterase: Not more than 1,0 to 10²%. Michaelis Constant: 4,3×10⁻⁵ M (cholesterol). Inhibitors: Ionic detergents, Hg²⁺, Ag⁺; Optimum Temperature: 45 to 50 °C; thermal stability: below 45 °C (pH 7,0; 15 minutes).