TransIT®-Jurkat reagent is specifically designed to provide exceptional transfection efficiency of plasmid DNA in Jurkat cells and cell types of associated lineage. Suitable for many applications such as gene expression and promoter analysis.

• High transfection efficiency (5 to 10%)
• Suitable for both transient and stable transfection
• Deliver single or multiple plasmids

Jurkat cells (human T-lymphocyte; acute T-cell leukemia cell line) have been inherently difficult to transfect, yet have remained a prevalent cell line in the immunological research field. This reagent helps to maximise transfection performance in Jurkat cells and other hard-to-transfect cells such as K562, RAW 264.7, THP-1 cells. The reagent also works well for additional cell lines of hematopoietic origin such as THP-1(human acute monocytic leukemia cell line) and RAW264.7 (mouse monocyte/macrophage cell line). Transfection with TransIT®-Jurkat reagent does not require medium changes and can be carried out in serum-containing medium.